Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

“…Compared with the control group, no new peaks were observed under the addition of 3.0% methionine ( Figure 4 b), suggesting that the addition of 3.0% methionine did not evidently affect the FTIR spectrum of BC. This finding was consistent with the result of a previous study [ 18 ], where BC membrane was produced in an optimized medium supplemented with amino acids. The signal bands around 900–1100 cm −1 became weaker with the addition of 3.0% methionine, which may be related to the stretching vibration of C–O and C–C from differential metabolites in FCW medium with and without the addition of methionine.…”

“…The crystallinity degree of the BC membrane obtained with the addition of 3.0% methionine was 7.0% higher than that in the control group. A high crystallinity was accompanied with a high yield of BC under the addition of 3.0% methionine, which was consistent with the study in a standard HS medium [ 18 ]. The mechanical property of BC might be enhanced under high crystallinity, which could increase the strength and durability of paper and is beneficial for the application of papermaking (e.g., the production of special paper) [ 41 ].…”

“…These results reflected that the exogenous addition of different concentrations of amino acids had varied effects on the yield of BC. Gomes et al [ 18 ] showed that the addition of aspartic acid, phenylalanine, and serine had a significant positive effect on the production of BC in a static culture condition. Son et al [ 33 ] found that the addition of tyrosine, valine, methionine, isoleucine, or glycine decreased the BC yield from Acetobacter sp.…”

“…When organic nitrogen is used, amino acids participate in the regulation of the activity of enzymes involved in the aminoacyl-tRNA synthesis pathway, thereby affecting the synthesis of proteins and a series of catalytic reactions in living cells [ 16 ]. The growth of microorganisms and the formation of metabolites could be improved in certain specific amino acids, whereas inappropriate amino acids perform the opposite effects [ 17 , 18 ]. Gomes et al [ 18 ] found that the addition of amino acids had different effects on the production of BC.…”

“…The growth of microorganisms and the formation of metabolites could be improved in certain specific amino acids, whereas inappropriate amino acids perform the opposite effects [ 17 , 18 ]. Gomes et al [ 18 ] found that the addition of amino acids had different effects on the production of BC. Three among the 19 amino acids tested, including aspartic acid, phenylalanine, and serine, had more significant positive effects on the synthesis of BC.…”