ABSTRACT. We investigated the diet content and properties of blood and milk in 11 pairs of Japanese Black cows and their calves for the one month following delivery. Four pairs (group A) had been no cases of white diarrhea during the year prior to this study, and 7 pairs (group B) had been a high occurrence of white diarrhea in calves during the same period. Properties of dams' diet in groups A and B before the onset of white diarrhea in calves were as follows: TDN fullness rate 98 ± 2% vs. 110 ± 5%, DCP fullness rate 151 ± 2% vs. 200 ± 33%, and starch content 5 ± 2% vs. 14 ± 3%. Blood and milk samples were collected from cows weekly and at the onset of white diarrhea in calves. No calves in group A had white diarrhea, while 5 out of 7 calves in group B had the diarrhea in this study. All cows were fed mixed hay, but 3 out of 5 cows whose calves had white diarrhea had been additionally given 3.0-3.5 kg of corn-silage a day from 4-5 days before onset. In all cows in the white diarrhea development group, the fat content of milk increased by 2.94 ± 1.82% at the day of onset in comparison before. Serum TG and BUN, respectively, increased by 3.8 ± 1.3 mg/dl and 3.7 ± 2.0 mg/dl. Feeding cows a lowstarch diet and additional corn-silage may induce a transient increase in blood TG, BUN and milk fat, which may contribute to the occurrence of white diarrhea in calves. [5,6] were possible causes of non-infectious white diarrhea.We reported that Japanese Black grazing cows in whose calves white diarrhea occurred showed a negative energy balance, acceleration of fatty acid metabolism, and abnormal condition of the rumen [9]. Properties and components of the milk of these cows were differed slightly from those of cows whose calves did not develop white diarrhea [9]. An examination of the metabolic profile of Japanese Black cows showed that cows whose calves developed white diarrhea at a high incidence were fed a low starch diet during the lactation period, and that the capacity of the liver to excrete fats was low and the condition of the rumen was abnormal [11]. However, it is not yet known whether white diarrhea in calves is related to the magnitude of fullness in cows in terms of energy or starch uptake. The purpose of this study is to clarify the relation between changes in blood and milk in cows and development of white diarrhea in their calves.