NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis

Lemaître, Quentin I. B.; Bartsch, Natascha; Kouzel, Ian U.; Busengdal, Henriette; Richards, Gemma S.; Steinmetz, Patrick R. H.; Rentzsch, Fabian

doi:10.1038/s41467-023-39789-4

Cited by 3 publications

(1 citation statement)

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“…While the overall composition of the nervous system in Nematostella has been described [ 27 – 29 ], to date there are few isolated neural sub-types that have been fully characterized in terms of their molecular fingerprint. Tourniere et al [ 30 ] describe the distribution and close relationship between the pou4 -expressing N2 neurons and the cnidocytes, and provide evidence of subtypes that we identify here that express RFamide (N2.2), glutamate receptor GRIK2-like-1 (NVE22966; N2.g1: also described here: [ 31 ]), and GABA-A receptor ( GBRB3-like-2 ; NVE21438), which here is expressed in the presumed early N2 cell state (N2.early, SoxB [ 2 ] expressing). Further, the same authors also provide characterization of the N1 subset [ 32 ] but do not identity specific neural sub-types.…”

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confidence: 60%

Updated single cell reference atlas for the starlet anemone Nematostella vectensis

Cole,

Steger,

Hagauer

et al. 2024

Front Zool

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Background The recent combination of genomics and single cell transcriptomics has allowed to assess a variety of non-conventional model organisms in much more depth. Single cell transcriptomes can uncover hidden cellular complexity and cell lineage relationships within organisms. The recent developmental cell atlases of the sea anemone Nematostella vectensis, a representative of the basally branching Cnidaria, has provided new insights into the development of all cell types (Steger et al Cell Rep 40(12):111370, 2022; Sebé-Pedrós et al. Cell 173(6):1520–1534.e20). However, the mapping of the single cell reads still suffers from relatively poor gene annotations and a draft genome consisting of many scaffolds. Results Here we present a new wildtype resource of the developmental single cell atlas, by re-mapping of sequence data first published in Steger et al. (2022) and Cole et al. (Nat Commun 14(1):1747, 2023), to the new chromosome-level genome assembly and corresponding gene models in Zimmermann et al. (Nat Commun 14, 8270 (2023). https://doi.org/10.1038/s41467-023-44080-7). We expand the pre-existing dataset through the incorporation of additional sequence data derived from the capture and sequencing of cell suspensions from four additional samples: 24 h gastrula, 2d planula, an inter-parietal region of the bodywall from a young unsexed animal, and another adult mesentery from a mature male animal. Conclusion Our analyses of the full cell-state inventory provide transcriptomic signatures for 127 distinct cell states, of which 47 correspond to neuroglandular subtypes. We also identify two distinct putatively immune-related transcriptomic profiles that segregate between the inner and outer cell layers. Furthermore, the new gene annotation Nv2 has markedly improved the mapping on the single cell transcriptome data and will therefore be of great value for the community and anyone using the dataset.

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Section: Resultssupporting

confidence: 60%

Updated single cell reference atlas for the starlet anemone Nematostella vectensis

Cole,

Steger,

Hagauer

et al. 2024

Front Zool

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A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone

Miramón-Puértolas,

Pascual-Carreras,

Steinmetz

2024

Nat Commun

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Germline segregation, essential for protecting germ cells against mutations, occurs during early embryogenesis in vertebrates, insects and nematodes. Highly regenerative animals (e.g., cnidarians), however, retain stem cells with both germinal and somatic potentials throughout adulthood, but their biology and evolution remain poorly understood. Among cnidarians (e.g., sea anemones, jellyfish), stem cells are only known in few hydrozoans (e.g., Hydra). Here, we identify and characterize a rare, multipotent population of stem and/or progenitor cells expressing the conserved germline and multipotency proteins Vasa2 and Piwi1 in the sea anemone Nematostella vectensis. Using piwi1 and vasa2 transgenic reporter lines, we reveal that the Vasa2+/Piwi1+ cell population generates not only gametes, but also a diversity of proliferative somatic cells, including neural progenitors, in juveniles and adults. Our work has uncovered a multipotent population of Vasa2+/Piwi1+ stem/progenitor cells that forms the cellular basis to understand body plasticity and regenerative capacities in sea anemones and corals.

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Aboral cell types ofClytiaand coral larvae have shared features and link taurine to the regulation of settlement

Ramon-Mateu,

Ferraioli,

Teixidó

et al. 2024

Preprint

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Planktonic larvae of many marine invertebrates settle on a suitable substrate and metamorphose into bottom-dwelling adults. Larval settlement is of considerable interest both for ecologists and for evolutionary biologists, who have proposed that anterior sensory systems for substrate selection provided the basis for animal brains. Nevertheless the cellular and molecular regulation of larval settlement, including in Cnidaria (corals, jellyfish, sea anemones, hydroids) is not well understood. We generated and compared anterior (aboral) transcriptomes and single-cell RNA-seq datasets from the planula larvae of three cnidarian species: the hydrozoan jellyfish Clytia hemisphaerica, and the scleractinian corals Astroides calycularis and Pocillopora acuta. Integrating these datasets and characterizing aboral cell types, we defined a common cellular architecture of the planula aboral end, and identified clade-specific specializations in cell types, including unique aboral neural cells in the Clytia planula and neurosecretory cell types with distinct molecular signatures in both Clytia and coral planulae. Among common planula aboral features were genes implicated in taurine uptake and catabolism expressed in distinct specialized cell types. In functional assays, exogenous taurine inhibited settlement of both Clytia and Astroides planulae. These findings define a detailed molecular and cellular framework of the planula aboral pole, and implicate localized taurine destruction in defining settlement competence.

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NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis

Cited by 3 publications

References 100 publications

Updated single cell reference atlas for the starlet anemone Nematostella vectensis

Updated single cell reference atlas for the starlet anemone Nematostella vectensis

A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone

Aboral cell types ofClytiaand coral larvae have shared features and link taurine to the regulation of settlement

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