NWD1 facilitates synaptic transmission and contributes to neuropathic pain

Wu, Yiling; Fu, Qiaochu; Huang, Xiaoxia; Luo, Yifan; Wan, Shengjun; Peng, Minjing; Su, Shanchun; Xu, Xueqin; Li, Yang; Li, Xiaohui; Sun, Dongsheng; Ke, Changbin

doi:10.1016/j.neuropharm.2021.108919

Cited by 4 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synaptic plasticity underlies spinal cord sensitization in NP, and synaptic transmission can be enhanced by synaptogenic signaling pathways, which are closely related to NP onset. 17 CAMP-mediated signaling in the spinal cord may be critical for the development of painful neuropathy, 18 and the autophagic pathway is a potential therapeutic target for NP. 19 These pathways mainly involve differentially expressed proteins, such as AGO2, AKT3, ATF2, and CAMK2A.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of the Spinal Dorsal Horn in Mice with Neuropathic Pain After Exercise

Bai¹,

Zhang²,

Zhou³

et al. 2023

JPR

View full text Add to dashboard Cite

Purpose Neuropathic pain (NP) is a chronic pain state with a complex etiology that currently lacks effective treatment in clinical practice. Studies have found that exercise training can alleviate NP hyperalgesia, but the specific mechanism remains unclear. Here, we sought to identify proteins and signaling pathways critical for mediating the effects of treadmill training on NP in a mouse model of spared nerve injury (SNI). Methods We used Tandem Mass Tag (TMT) technology for proteins and signaling pathways identification. Functional enrichment analyses were conducted using DAVID and Metascape software. Ingenuity pathway analysis was used to conduct functional annotation and analyze alterations in canonical pathways and molecular networks. Reverse transcription quantitative PCR (RT-qPCR) was used to confirm the results of proteomics analysis. Results A total of 270 differentially expressed proteins were screened in the detrained and trained groups ( P ≤0.05). Enrichment and ingenuity pathway analysis revealed the effects of treadmill training on autophagy, cAMP-mediated signaling, calcium signaling and NP signaling in dorsal horn nerves. Treadmill training reduced the expression of Akt3, Atf2, Gsk3b, Pik3c3, Ppp2ca , and Sqstm1 , and increased the expression of Pik3cb in the autophagic pathway. Conclusion Our results suggest that treadmill training may alleviate nociceptive hyperalgesia in NP mice by modulating the autophagic pathway, providing unique mechanistic insights into the analgesic effects of exercise.

show abstract

Section: Discussionmentioning

confidence: 99%