NYC Medium for Simultaneous Isolation of Neisseria gonorrhoeae , Large-Colony Mycoplasmas, and T-Mycoplasmas

Abstract

NYC medium, primarily designed for isolation of pathogenic Neisseria , also readily supports the growth of large-colony mycoplasmas and T-mycoplasmas. When compared with A-3 agar medium and PPLO agar in clinical field trials, NYC medium performed equally as well as these mycoplasma-specific media in providing recovery of Mycoplasma species from female genital specimens. The transparent, highly-selective NYC medium permits direct, microscopic observation and presumptive identification of mycoplasmas, as well as Neisseria gonorrhoeae , without interference from contaminating saprophytes. As a single medium it can therefore be doubly useful in the diagnosis of gonorrhea and in the recognition of active or asymptomatic mycoplasma infections. Used in gonorrhea screening programs, the medium can be valuable in establishing the frequency of association of mycoplasmas with urogenital tract infection.

Field evaluation of New York City medium in the biological environment-CO2 chamber in recovery of Neisseria gonorrhoeae and urogenital mycoplasmas

“…(3, 4). It also readily supports the growth of large-colony mycoplasmas and T-mycoplasmas from urogenital specimens (6). At the time of its original formulation, NYC medium, with the addition of yeast dialysate filtrate, was evaluated as a transport system by using it in closed vessels such as screw-capped plates and screw-capped bottles (5).…”

“…Laboratory evaluation of the CO2 culture system. The selective ability of NYC medium in the CO2 culture-transport system (NYC-BEC) to support the growth of freshly isolated strains of pathogenic Neisseria, large-colony mycoplasmas, and T-mycoplasmas was evaluated as previously described (6). Large-colony mycoplasmas and T-strain mycoplasmas were transferred from isolation plates to NYC-BEC by the agar block method.…”