Nyctinastic thallus movement in the liverwort<i>Marchantia polymorpha</i>is regulated by a circadian clock

Lagercrantz, Ulf; Billhardt, Anja; Rousku, Sabine; Eklund, D. Magnus

doi:10.1101/2020.02.09.940403

Cited by 3 publications

(18 citation statements)

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“…First, we co‐transformed sporelings with EF1 pro :amiR‐ Mp EFL MpMIR160 and a bioluminescence reporter for the Mp PRR promoter (Mp PRR pro : LUC ; Linde et al ., 2017) to complement previous studies on Mp LUX by testing the role of Mp EFL in regulation of the circadian clock. We observed fast damping of nyctinastic movements and Mp PRR pro : LUC signals, as well as elevated Mp PRR expression with attenuated amplitude, in the amiR‐ Mp EFL Mp MIR160 Mp PRR pro : LUC lines in LL, similar to previous observations for Mp lux ge (Figs S3, 3c; Lagercrantz et al ., 2020). Subsequently we used a bioluminescence approach to study the activity of a 4.4‐kb Mp DET1 promoter fragment in knock‐down plants of Mp LUX and Mp EFL .…”

Section: Resultsmentioning

confidence: 99%

“…This sampling has been described before (Lagercrantz et al, 2020). Growth and four-h-interval sampling of Tak-1 and the Mpprr ko mutant are also described in Lagercrantz et al (2020).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted at eight time points, with three replicates per time point, using an RNeasy Plant Mini Kit (Qiagen) with on‐column DNase treatment. We extracted RNA at a stage of development at which all organs and tissue types of the thallus are produced, except for gemma cups, and where the thallus apex displayed nyctinastic movements (Lagercrantz et al ., 2020). Sampling was conducted at 6‐h intervals without disturbing the light regime.…”

Section: Methodsmentioning

confidence: 99%

“…Marchantia polymorpha DET1 expression is modulated in mutants of core clock genes Because MpDET1 expression is rhythmic under ND, LL and DD conditions, we asked if core circadian clock components described by Linde et al (2017) and Lagercrantz et al (2020) could modulate the expression of MpDET1. As seen in Fig.…”

Section: Marchantia Polymorpha Contains a Single Det1 Homologue With Circadian Expression Patternmentioning

confidence: 99%

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DE‐ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha

et al. 2021

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Previous studies of plant circadian clock evolution have often relied on clock models and genes defined in Arabidopsis. These studies identified homologues with seemingly conserved function, as well as frequent gene loss. In the present study, we aimed to identify candidate clock genes in the liverwort Marchantia polymorpha using a more unbiased approach.To identify genes with circadian rhythm we sequenced the transcriptomes of gemmalings in a time series in constant light conditions. Subsequently, we performed functional studies using loss-of-function mutants and gene expression reporters.Among the genes displaying circadian rhythm, a homologue to the transcriptional corepressor Arabidopsis DE-ETIOLATED1 showed high amplitude and morning phase. Because AtDET1 is arrhythmic and associated with the morning gene function of AtCCA1/LHY, that lack a homologue in liverworts, we functionally studied DET1 in M. polymorpha.We found that the circadian rhythm of MpDET1 expression is disrupted in loss-of-function mutants of core clock genes and putative evening-complex genes. MpDET1 knock-down in turn results in altered circadian rhythm of nyctinastic thallus movement and clock gene expression. We could not detect any effect of MpDET1 knock-down on circadian response to light, suggesting that MpDET1 has a yet unknown function in the M. polymorpha circadian clock.

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Section: Resultsmentioning

confidence: 99%

“…This sampling has been described before (Lagercrantz et al, 2020). Growth and four-h-interval sampling of Tak-1 and the Mpprr ko mutant are also described in Lagercrantz et al (2020).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Marchantia Polymorpha Contains a Single Det1 Homologue With Circadian Expression Patternmentioning

confidence: 99%

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DE‐ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha

et al. 2021

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“…1c,d). Molecular studies of the liverwort Marchantia polymorpha revealed characteristics of a circadian oscillator, as gene expression and thallus movement were rhythmic under constant light (Lagercrantz et al ., 2020). However, they appear to have lost the CCA1/LHY/RVE homolog and instead possess a single RVE3,4,5,6,8‐like homolog (Linde et al ., 2017) (Fig.…”

Section: The Origins Of Clock‐mediated Abiotic Stress Response In the Green Lineagementioning

confidence: 99%

Abiotic stress through time

Markham

Greenham

2021

New Phytologist

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Throughout plant evolution the circadian clock has expanded into a complex signaling network, coordinating physiological and metabolic processes with the environment. Early land plants faced new environmental pressures that required energy-demanding stress responses. Integrating abiotic stress response into the circadian system provides control over daily energy expenditure. Here, we describe the evolution of the circadian clock in plants and the limited, yet compelling evidence for conserved regulation of abiotic stress. The need to introduce abiotic stress tolerance into current crops has expanded research into wild accessions and revealed extensive variation in circadian clock parameters across monocot and eudicot species. We argue that research into the ancestral links between the clock and abiotic stress will benefit crop improvement efforts.

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Circadian and diel regulation of photosynthesis in the bryophyte Marchantia polymorpha

Cuitun‐Coronado

Rees

Hall

et al. 2022

Preprint

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Circadian rhythms are 24-hour biological cycles that align metabolism, physiology and development with daily environmental fluctuations. Photosynthetic processes are governed by the circadian clock in both flowering plants and cyanobacteria, but it is unclear how extensively this is conserved throughout the green lineage. We investigated the contribution of circadian regulation to photochemistry in Marchantia polymorpha, a liverwort that diverged from flowering plants early in the evolution of land plants. First, we identified in M. polymorpha the circadian regulation of several measures of photosynthetic biochemistry (delayed fluorescence, the rate of photosynthetic electron transport, and non-photochemical quenching of chlorophyll fluorescence). Second, we identified that light-dark cycles increase the robustness of the 24 h cycles of photosynthesis in M. polymorpha, which might be due to the masking of underlying circadian rhythms of photosynthesis by light-dark cycles. Finally, we used a pharmacological approach to identify that chloroplast translation might be necessary for clock control of light harvesting in M. polymorpha. We infer that the circadian regulation of photosynthesis might be well-conserved amongst terrestrial plants.

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Nyctinastic thallus movement in the liverwortMarchantia polymorphais regulated by a circadian clock

Cited by 3 publications

References 39 publications

DE‐ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha

DE‐ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha

Abiotic stress through time

Circadian and diel regulation of photosynthesis in the bryophyte Marchantia polymorpha

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