Nylon adherent antigen-specific rosette-forming T cells.

Cone, Robert E.; Gershon, Richard K.; Askenase, P W

doi:10.1084/jem.146.5.1390

Cited by 27 publications

(10 citation statements)

References 20 publications

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“…In one experiment, however, in which Fc-receptor-bearing cells were strongly reduced, considerable loss of T cells was also observed. This effect was possibly caused by loss of activated T cells which are thought to carry Fc-receptors [21] and to have adherence properties [2]. In contrast to Fernandez and MacSween [5] who found significantly enriched T cells af ter Cl treatment, our results show propor tional increase of a lymphocyte-like cell type, different from B and T lymphocytes [6], as detectable by surface immunofluo rescence and SRBC-rosette formation.…”

Section: Resultscontrasting

confidence: 88%

Influence of Carbonyl Iron Treatment on Lymphocyte Subpopulations

Rumpold¹,

D²,

Scheiner³

et al. 1979

Int Arch Allergy Immunol

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Section: Resultscontrasting

confidence: 88%

Influence of Carbonyl Iron Treatment on Lymphocyte Subpopulations

Rumpold¹,

D²,

Scheiner³

et al. 1979

Int Arch Allergy Immunol

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“…The in vitro properties of RFCs in the unseparated populations appear to support the assumption that the RFC represent antigen-binding B cells which are precursors for specific RFCs. The fai lure to show antigen binding by T cells in the form of RFCs stable enough to resist the mechanical proce dures involved during rosette separation is in accor dance with studies carried out in other species [22].…”

Section: Discussionsupporting

confidence: 65%

The Appearance of Specific Helper Cells in Lymph of Immunized Sheep

Heron¹,

Cahill²,

Trnka³

1982

Int Arch Allergy Immunol

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Culture conditions which support the induction by antigen of plaque-forming cells (PFCs) in cell suspensions made from lymph nodes from sheep do not support the induction of PFCs in suspensions of non-primed lymphocytes obtained from the efferent lymph of a lymph node. The lymphocytes in the efferent lymph of a node after immunization with heterologous erythrocyte antigen, via an afferent lymphatic vessel, however, respond in vitro to the specific antigen with the generation of PFCs. Immune responses were confined to single lymph nodes, and the lymphocytes of the efferent lymph issuing from these were added to nonimmunized efferent lymph cells of other nodes in the same sheep. It was found that the addition of low numbers of immunized T lymphocytes to saturating numbers of nonimmune lymphocytes, either unseparated, or the B cell fraction only, gave rise in cultures stimulated with the specific antigen to PFCs in numbers comparable to those induced in cultures containing only saturating amounts of immunized lymph node lymphocytes. Immunized B cells were not capable of providing help. The specific helper T cells required were found to be recirculating between blood and lymph for several months after immunization. Irradiated T cells were not capable of providing sufficient help. The number of lymphocytes forming rosettes with chicken red blood cells or horse red blood cells in efferent lymph increases after immunization of a sheep lymph node through an afferent lymphatic vessel with specific antigens. Rosette-forming cells (RFCs) were removed by Ficoll-Isopaque flotation and the properties of the RFCs were determined in the culture system. It was found that specific B precursor cells were antigen binding because the response in vitro was abolished after these rosette-forming B cells had been removed. Helper T cells appearing in the efferent lymph subsequent to immunization were found not to be antigen binding because T cells issuing from immunized lymph nodes, after removal of RFCs, were still capable of providing specific help to a nonimmunized B cell population.

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“…In the actively sensitized animals challenged at 4 d, there is little evidence for B cell activation as measured by serum antibody (4), plaque-forming cells (16), or antigenbinding rosette-forming B cells (6). Moreover, mice that are treated with anti-mu sera and are rendered agammaglobulinemic and unable to make antibody responses have intact DTH (17,18).…”

Section: T Cell Dependence Of Mast Cell Activation and Endothelial Gamentioning

confidence: 99%

“…The harvest of spleen cells, fractionation on nylon-wool columns, cell transfers of DTH, and treatment with cyclophosphamide were done as described previously (4)(5)(6).…”

mentioning

confidence: 99%

T cell-dependent mast cell degranulation and release of serotonin in murine delayed-type hypersensitivity.

Askenase¹,

Bursztajn²,

Gershon³

et al. 1980

The Journal of Experimental Medicine

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160

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In a previous study of murine delayed-type hypersensitivity (DTH) 1 we suggested that the release of serotonin (5-hydroxytryptamine) (5-HT) by local tissue mast cells was required for the elicitation of these responses (1). We noted that DTH was preferentially elicited in those cutaneous sites that were especially rich in 5-HTcontaining mast cells, and that administration of the 5-HT-depleting agent, reserpine, abolished the ability to elicit DTH. Because the effect of reserpine could be prevented by administration of a monoamine oxidase (MAO) inhibitor, the attribution of the action of reserpine to depletion of a monoamine (such as 5-HT) was confirmed (1). Subsequent studies, which have employed two additional and independent pharmacological maneuvers (5-HT tachyphylaxis of the vasculature [2] and the use of 5-HT antagonists2), have been consistent with our hypothesis that in murine DTH, specifically sensitized T cells interact with tissue mast ceils leading to the release of 5-HT, which acts on the local endothelium allowing the emigration of bone marrow-derived leukocytes from the intravascular to the extravascular space (3). The current study was undertaken to test our hypothesis by determining if we could obtain direct evidence for activation of mast cells to release 5-HT during murine DTH. Immunization and Skin Testing (Challenge). BDFI mice (The Jackson Laboratory, Bar Harbor, Maine) received an optimal immunization for the eticitation of DTH (0.2 ml of 0.01% sheep erythrocytes [SRBC]; [Colorado Serum Labs, Boulder, Colo.] diluted in sterile saline) (4). 4 d later, the animals were tested for DTH by injecting 0.03 ml of 20% SRBC into the ventral surface of a rear footpad. The thickness of the footpad was measured with a micrometer before and after challenge with antigen, and the percent change calculated (5). Immunized controls were challenged by footpad injection of 0.03 ml saline. Nonimmunized controls received an intravenous injection of saline on day 0 and were tested by footpad injection of 20% SRBC on day 4. In some experiments, contact hypersensitivity reactions were elicited in the skin of the * Materials and Methods

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Nylon adherent antigen-specific rosette-forming T cells.

Cited by 27 publications

References 20 publications

Influence of Carbonyl Iron Treatment on Lymphocyte Subpopulations

Influence of Carbonyl Iron Treatment on Lymphocyte Subpopulations

The Appearance of Specific Helper Cells in Lymph of Immunized Sheep

T cell-dependent mast cell degranulation and release of serotonin in murine delayed-type hypersensitivity.

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