Nylon wool purification alters the activation of T cells

Wohler, Jillian E.; Barnum, Scott R.

doi:10.1016/j.molimm.2008.09.017

Cited by 16 publications

(12 citation statements)

References 9 publications

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“…CD4 levels have also been shown to be relatively low in sea bass intestine, compared to CD8α expression [22]. Since protocol-1 used nylon wool columns that could impact on yield and cell activity/ gene expression, as seen in mice [47,48], this step was excluded in protocol-2. This significantly increased the cell yield, to 6.4x10 6 cells /fish, with no effect on cell viability or LPS responsiveness, but did result in a higher relative expression of some dendritic cell markers.…”

Section: Discussionmentioning

confidence: 99%

Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines

Attaya

Wang

Zou

et al. 2018

Fish & Shellfish Immunology

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Increased knowledge of the immune response of the intestine, a physiologically critical organ involved in absorption, secretion and homeostasis in a non-sterile environment, is needed to better understand the mechanisms involved in the induction of long-lasting immunity and, subsequently, the development of efficacious gastrointestinal immunization approaches. To this end, analysis of isolated gut cells will give an insight into the cell types present and their immune capability. Hence, in this study we first optimised a method for salmonid gut leucocyte isolation and characterised the cells on the basis of their expression of a range of selected cell markers associated with T & B cells and dendritic cells. The GALT leucocytes were then stimulated with a variety of PAMPs, recombinant cytokines and PHA, as a means to help characterise the diversity of the immune repertoire present in such cells. The stimulants tested were designed to examine the nature of the antibacterial, antiviral and T cell type responses in the cells (at the transcript level) using a panel of genes relevant to innate and adaptive immunity. The results showed distinct responses to the stimulants, with a clear delineation seen between the stimulant used (eg viral or bacterial PAMP) and the pathway elicited. The changes in the expression patterns of the immune genes in these cells indicates that the salmonid intestine contains a good repertoire of competent immune cells able to respond to different pathogen types. Such information may aid the development of efficient priming by oral vaccination in salmonids.

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Section: Discussionmentioning

confidence: 99%

Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines

Attaya

Wang

Zou

et al. 2018

Fish & Shellfish Immunology

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“…It has been shown that this can reduce T-cell proliferation and may lead to altered production of cytokines (40). However, the possible influence of this factor would remain constant throughout the T-cell experiments.…”

Section: Discussionmentioning

confidence: 99%

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Ilves¹,

Harvima²

2015

Acta Derm Venerol

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Mast cell chymase and interleukin (IL)-6 can be involved in atopic dermatitis and chymase can degrade IL-6. Our aim was therefore to study the expression of mast cell chymase and IL-6 in atopic skin using enzyme- and immunohistochemistry and to analyse their interaction in vitro. Chymase activity was significantly reduced in mast cells in skin lesions whereas the percentage of IL-6(+) mast cells was increased. Low recombinant human (rh)-chymase concentration (10-100 ng/ml) stimulated and higher concentration (1,000 ng/ml) inhibited the proliferation of T cells and peripheral blood mononuclear cells. Rh-IL-6 inhibited T-cell proliferation, and even inhibited the proliferation induced by rh-chymase. Pretreatment of rh-IL-6 with a high rh-chymase concentration prevented the IL-6-induced inhibition in T-cell proliferation. The results suggest that reduction in chymase activity can give rise to increased cellular effects of mast cell IL-6 and that chymase and IL-6 can modify each other's effects.

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“…1E, F). In one study, T-cell enrichment by nylon wool was shown to alter T-cell activation status through decreased cytokine production (58). Appropriate controls in each experiment were designed (T cells alone) to correct for any baseline activation.…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells

et al. 2012

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Mesenchymal stem cells (MSCs) derived from bone marrow (BM), adipose tissue (AT), umbilical cord blood (CB), and umbilical cord tissue (CT) are increasingly being used to treat equine inflammatory and degenerative lesions. MSCs modulate the immune system in part through mediator secretion. Animal species and MSC tissue of origin are both important determinants of MSC function. In spite of widespread clinical use, how equine MSCs function to heal tissues is fully unknown. In this study, MSCs derived from BM, AT, CB, and CT were compared for their ability to inhibit lymphocyte proliferation and secrete mediators in response to activation. Five MSC lines from each tissue were isolated. Lymphocyte proliferation was assessed in a mixed leukocyte reaction, and mediator secretion was determined by ELISA. Regardless of tissue of origin, quiescent MSCs did not alter lymphocyte proliferation or secrete mediators, except for transforming growth factor-β (TGF-β1). When stimulated, MSCs of all tissue types decreased lymphocyte proliferation, increased prostaglandin (PGE2) and interleukin-6 (IL-6) secretion, and decreased production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). BM-MSCs and CB-MSCs also produced nitric oxide (NO), while AT-MSCs and CT-MSCs did not. Equine MSCs did not produce indoleamine 2,3-dioxygenase (IDO). These data suggest that activated equine MSCs derived from BM, AT, CT, and CB secrete high concentration of mediators and are similar to MSCs from rodents and humans in their immunomodulatory profiles. These findings have implication for the treatment of inflammatory lesions dominated by activated lymphocytes and TNF-α and IFN-γ in vivo.

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Nylon wool purification alters the activation of T cells

Cited by 16 publications

References 9 publications

Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines

Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells

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