NZF-2b is a novel predominant form of mouse NZF-2/MyT1, expressed in differentiated neurons especially at higher levels in newly generated ones

Matsushita, Fumio; Kameyama, Tetsuya; Marunouchi, Tohru

doi:10.1016/s0925-4773(02)00250-2

Cited by 23 publications

(37 citation statements)

References 11 publications

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“…These two isoforms differ in their Nterminal 100 amino acid residues (Matsushita et al, 2002), such that NZF2b has an extra zinc finger (MYT1a has 6 zinc fingers and NZF2b has 7 zinc fingers). For simplicity, we refer to both RNA isoforms from the Myt1 locus as Myt1, and we refer to the 6-zinc-finger Myt1 cDNA as Myt1a (Kim et al, 1997a;Matsushita et al, 2002). Our semi-quatitative RT-PCR results showed that Myt1a and Nzf2b are both expressed in the developing pancreas, with Nzf2b being expressed at much higher levels (data not shown).…”

Section: Myt1mentioning

confidence: 99%

Global expression analysis of gene regulatory pathways during endocrine pancreatic development

et al. 2004

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To define genetic pathways that regulate development of the endocrine pancreas, we generated transcriptional profiles of enriched cells isolated from four biologically significant stages of endocrine pancreas development:endoderm before pancreas specification, early pancreatic progenitor cells,endocrine progenitor cells and adult islets of Langerhans. These analyses implicate new signaling pathways in endocrine pancreas development, and identified sets of known and novel genes that are temporally regulated, as well as genes that spatially define developing endocrine cells from their neighbors. The differential expression of several genes from each time point was verified by RT-PCR and in situ hybridization. Moreover, we present preliminary functional evidence suggesting that one transcription factor encoding gene (Myt1), which was identified in our screen, is expressed in endocrine progenitors and may regulate α, β andδ cell development. In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation.

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Section: Myt1mentioning

confidence: 99%

Global expression analysis of gene regulatory pathways during endocrine pancreatic development

et al. 2004

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“…As for the last alternative, in situ expression data indicate that the MyT1 isoforms are expressed in newly generated neurons throughout the CNS, including RGCs. Thus, the regulation of MyT1 expression could then allow newly generated cells to escape from Notch inhibition [Matsushita et al, 2002]. Further experimentation will be necessary to determine the respective contributions of these mechanisms in mediating the inhibition of Notch activity in differentiating ganglion cells.…”

Section: Downregulation Of Notch Activity Drives Early Progenitor Celmentioning

confidence: 99%

Notch Activity Is Downregulated Just prior to Retinal Ganglion Cell Differentiation

Nelson¹,

Gümüşçü²,

Hartman

et al. 2006

Dev Neurosci

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The Notch signaling pathway is important at several stages of retinal development including the differentiation of retinal ganglion cells and Müller glia. The downstream effectors of Notch signaling, Hes1 and Hes5, have been shown to be critical in the retina as well. While Notch activity directly regulates Hes1 and Hes5 elsewhere in the nervous system, it has been unclear whether Hes1 and/or Hes5 are directly regulated by Notch activity in the developing retina. Here, we report that both Hes1 and Hes5 are directly regulated by Notch activity during retinal development. Using fluorescence-based Hes1 and Hes5 reporter constructs, we can monitor Notch activity in progenitor cells in the intact retina, and we find that Notch activity is downregulated just prior to retinal ganglion cell differentiation.

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“…Both Myt1 and Myt1L proteins reside in distinct domains within the neuronal and oligodendrocyte nuclei, expressed as discrete patterns (Armstrong et al 1995;Kim et al, 1997) and they interact with the corepressor Sin3B and actively recruit HDAC1 and HDAC2 to specific genes (Romm et al, 2005). The NZF-2/Myt1 comprises two isoforms, the poorly expressed NZF-2a/Myt1 (6-zinc finger form) and the predominant NZF-2b/7ZFMyt1 (7-zinc-finger form), located on chromosome 20 and 2 of human and mouse, respectively (Nagase et al, 1998;Kikuno et al, 1999;Matsushita et al, 2002;Deloukas et al, 2002;Nielsen et al, 2004). But the role of these two isoforms in neurons is poorly known.…”

Section: Introductionmentioning

confidence: 99%

The brain-specific Neural Zinc Finger transcription factor 2b (NZF-2b/7ZFMyt1) causes suppression of cocaine-induced locomotor activity

Chandrasekar

Dreyer

2010

Neurobiology of Disease

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Chronic cocaine induces high expression of the brain-specific Neural-Zinc-Finger transcription factor-2b (NZF-2b/7ZFMyt1), particularly in the mesolimbic dopaminergic pathway, resulting in a 11-fold increase in NZF-2b/7ZFMyt1 expression in the Nucleus Accumbens (NAc). Overexpression of this gene in the NAc with a NZF-2b/7ZFMyt1-expressing lentivirus resulted in N55% decrease in locomotor activity upon chronic cocaine administration, compared to control animals. In contrast knocking-down the gene in the NAc with lentiviruses expressing shRNAs against NZF-2b/7ZFMyt1 induced strong hyperlocomotor activity upon cocaine. Strong inhibition of BDNF is observed upon NZF-2b/7ZFMyt1 expression, concomitant with strong induction of transcription factors REST1 (RE silencing transcription factor-1) and NAC1, probably leading to regulation of gene expression by interaction with histone deacetylases. These changes lead to decreased responsiveness of the animal to the locomotor-activating effects of cocaine, indicating that NZF-2b/7ZFMyt1 expression plays an important role in phenotypic changes induced by the drug.

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NZF-2b is a novel predominant form of mouse NZF-2/MyT1, expressed in differentiated neurons especially at higher levels in newly generated ones

Cited by 23 publications

References 11 publications

Global expression analysis of gene regulatory pathways during endocrine pancreatic development

Global expression analysis of gene regulatory pathways during endocrine pancreatic development

Notch Activity Is Downregulated Just prior to Retinal Ganglion Cell Differentiation

The brain-specific Neural Zinc Finger transcription factor 2b (NZF-2b/7ZFMyt1) causes suppression of cocaine-induced locomotor activity

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