O-17. PGD: application of the ‘mini-sequencing method’

Fiorentine, F; Magli, Christina; Podini, Daniele; Nuccitelli, Annalisa; Ferraretti, AP; Baldi, Marina; Gianaroli, Luca

doi:10.1016/s1472-6483(12)60036-4

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2011

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“…Solid phase minisequencing, a powerful single base detection method based on DNA microarray, 1 is widely used in DNA mutation detection, 2-5 single nucleotide polymorphisms (SNPs) genotyping, [6][7][8][9][10][11] messenger RNA (mRNA) alternative splicing studies, 12 13 single gene disorders research, 14 and single nucleotide mismatch detection. 15 After certain improvement, solid phase minisequencing method is also a useful sequencing-by-synthesis approach.…”

Section: Introductionmentioning

confidence: 99%

2-Color Encoding Solid Phase Minisequencing

Bai²,

Ge³

et al. 2011

J. Nanosci. Nanotech.

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Solid phase minisequencing is an effective single nucleotide determination technique. However, two main methods currently used are both time and money consuming. Here, we introduced a more economical and time efficient approach, called 2-color encoding minisequencing, in which four varieties of nucleotides were labeled by the combination of two fluorescence dyes. Such code was sensitive for different nucleotides, and the sequencing results were highly uniform and repeatable.

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