O-antigen and Core Carbohydrate of Vibrio fischeri Lipopolysaccharide

Post, Deborah M. B.; Yu, Liping; Krasity, Benjamin C.; Choudhury, Biswa; Mandel, Mark J.; Brennan, Caitlin A.; Ruby, Edward G.; McFall‐Ngai, Margaret J.; Gibson, Bradford W.; Apicella, Michael A.

doi:10.1074/jbc.m111.324012

Cited by 58 publications

(36 citation statements)

References 76 publications

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“…The addition of this LPS extract significantly inhibited biofilm growth in a concentration-dependent manner ( Figure 1B), as previously shown by Kim et al (2007). To further evaluate the inhibitory role of LPS in biofilm formation, the lipid moiety of LPS was removed by acidic hydrolysis (Post et al 2012). The resultant dLPS, which does not include the lipid A part of LPS ( Figure 1A, right lane), was added to the biofilm assay at time zero.…”

Section: Effects Of the Exogenous Addition Of Lps Dlps Lps Core Comsupporting

confidence: 69%

“…Since the LPS fraction was found to be slightly contaminated with another type of exopolysaccharide that is tightly bound to membranes, such as CPS, the wbpP mutant defective in CPS production (Lee et al 2013) was used to prepare CPS-free LPS. Lipid A components in the extracted LPS were removed by treating LPS with 1% acetic acid at 100°C for 2 h, as described by Post et al (2012). The resulting dLPS was precipitated with ethanol and resuspended in distilled water.…”

Section: Extraction and Deacylation Of Lpsmentioning

confidence: 99%

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Deacylated lipopolysaccharides inhibit biofilm formation by Gram-negative bacteria

Lee

Hwang

et al. 2016

Biofouling

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The extracellular polysaccharides of Vibrio vulnificus play different roles during biofilm development. Among them, the effect of lipopolysaccharide (LPS), which is crucial for bacterial adherence to surfaces during the initial stage of biofilm formation, on the formation process was examined using various types of LPS extracts. Exogenously added LPS strongly inhibited biofilm formation in a dose-dependent manner. In addition, the exogenous addition of a deacylated form of LPS (dLPS) also inhibited biofilm formation. However, an LPS fraction extracted from a mutant not able to produce O-antigen polysaccharides (O-Ag) did not have an inhibitory effect. Furthermore, biofilm formation by several Gram-negative bacteria was inhibited by dLPS addition. In contrast, biofilm formation by Gram-positive bacteria was not influenced by dLPS but was affected by lipoteichoic acid. Therefore, this study demonstrates that O-Ag in LPS is important for inhibiting biofilm formation and may serve an efficient anti-biofilm agent specific for Gram-negative bacteria.

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Section: Effects Of the Exogenous Addition Of Lps Dlps Lps Core Comsupporting

confidence: 69%

Section: Extraction and Deacylation Of Lpsmentioning

confidence: 99%

Deacylated lipopolysaccharides inhibit biofilm formation by Gram-negative bacteria

Lee

Hwang

et al. 2016

Biofouling

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“…In contrast to the ompU mutant, neither colonization by the waaL mutant nor treatment with waaL mutant-derived OMV resulted in a detectable difference in phenotypic responses from that of wild-type cells (see Fig. S2 in the supplemental material), even though the waaL mutant colonizes at a lower level (18). These results suggest that OMV recognition is not dependent on O-antigen recognition.…”

Section: Resultsmentioning

confidence: 82%

“…fischeri has been reported to express an unusual O antigen on its LPS, so we next asked whether this major envelope surface element plays a role in OMV recognition. Specifically, we determined how host development was affected by symbionts carrying a mutation in the waaL gene, which eliminates the bacterium's ability to ligate the O antigen to the core LPS (18). In contrast to the ompU mutant, neither colonization by the waaL mutant nor treatment with waaL mutant-derived OMV resulted in a detectable difference in phenotypic responses from that of wild-type cells (see Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, while Pseudomonas O-antigen mutants have no effect on the rate of OMV release, they do affect both their contents and size (43). The LPS of V. fischeri cells includes an unusual O antigen that plays a critical role in both flagellar motility and efficient colonization (18), but there is little understanding of this relationship or its relationship to broader changes in either the cell's surface or its OMVs. Exposure to LPS from any of several bacterial sources induces normal apoptosis in the ciliated fields of the light organ (7), suggesting that this host response is not specific to LPS expressed by V. fischeri or its unusual O antigen (27).…”

Section: Discussionmentioning

confidence: 99%

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Rotation of Vibrio fischeri Flagella Produces Outer Membrane Vesicles That Induce Host Development

et al. 2016

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Using the squid-vibrio association, we aimed to characterize the mechanism through which Vibrio fischeri cells signal morphogenesis of the symbiotic light-emitting organ. The symbiont releases two cell envelope molecules, peptidoglycan (PG) and lipopolysaccharide (LPS) that, within 12 h of light organ colonization, act in synergy to trigger normal tissue development. Recent work has shown that outer membrane vesicles (OMVs) produced by V. fischeri are sufficient to induce PG-dependent morphogenesis; however, the mechanism(s) of OMV release by these bacteria has not been described. Like several genera of both beneficial and pathogenic bacteria, V. fischeri cells elaborate polar flagella that are enclosed by an extension of the outer membrane, whose function remains unclear. Here, we present evidence that along with the well-recognized phenomenon of blebbing from the cell's surface, rotation of this sheathed flagellum also results in the release of OMVs. In addition, we demonstrate that most of the development-inducing LPS is associated with these OMVs and that the presence of the outer membrane protein OmpU but not the LPS O antigen on these OMVs is important in triggering normal host development. These results also present insights into a possible new mechanism of LPS release by pathogens with sheathed flagella. IMPORTANCEDetermining the function(s) of sheathed flagella in bacteria has been challenging, because no known mutation results only in the loss of this outer membrane-derived casing. Nevertheless, the presence of a sheathed flagellum in such host-associated genera as Vibrio, Helicobacter, and Brucella has led to several proposed functions, including physical protection of the flagella and masking of their immunogenic flagellins. Using the squid-vibrio light organ symbiosis, we demonstrate another role, that of V. fischeri cells require rotating flagella to induce apoptotic cell death within surface epithelium, which is a normal step in the organ's development. Further, we present evidence that this rotation releases apoptosis-triggering lipopolysaccharide in the form of outer membrane vesicles. Such release may also occur by pathogens but with different outcomes for the host.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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O-antigen and Core Carbohydrate of Vibrio fischeri Lipopolysaccharide

Cited by 58 publications

References 76 publications

Deacylated lipopolysaccharides inhibit biofilm formation by Gram-negative bacteria

Deacylated lipopolysaccharides inhibit biofilm formation by Gram-negative bacteria

Rotation of Vibrio fischeri Flagella Produces Outer Membrane Vesicles That Induce Host Development

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

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