O-Carboxymethyl-chitosan/organosilica hybrid nanoparticles as non-viral vectors for gene delivery

Zhang, Yafei; Yin, Ping; Zhao, Xueqin; Wang, Jūn; Wang, Jùn; Wang, Cai-ding; Ren, Lei; Zhang, Qiqing

doi:10.1016/j.msec.2009.04.003

Cited by 31 publications

(13 citation statements)

References 36 publications

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“…Figure 2 shows the viability data of preadipocytes. Chitosan and its two derivatives were proved to have a low cytotoxicity to preadipocytes, which was in agreement with published reports [30,35,36]. The cell viability of the two chitosan derivatives was slightly lower than that of chitosan.…”

Section: Cytotoxicity Analysissupporting

confidence: 90%

Therapeutic Effect of Carboxymethylated and Quanternized Chitosan on Insulin Resistance in High-Fat-Diet-Induced Rats and 3T3-L1 Adipocytes

Liu

Yang

Song

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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Owing to their distinct biochemical properties, chitosan and its derivatives have a great potential in a range of bioapplications. One such application is as a dietary antilipidemic supplement to be used in reducing obesity and to improve insulin resistance. The lipid-binding efficiency of chitosan and its derivatives, however, remains debatable. Accordingly, in this study we investigated the interaction of chitosan and its two derivatives, O-carboxymethyl chitosan (O-CMCs) and N-[(2-hydroxy-3-N,N-dimethylhexadecyl ammonium)propyl]chitosan chloride (N-CQCs), with plasma leptin, glucose, insulin and total cholesterol in a diet-induced insulin-resistant rat model, and further interaction with mRNA expression of adipocytokines and its related molecule PPAR-γ. The experiments were performed using the RT-PCR technique in cultured 3T3-L1 adipocytes, in which the mRNA expression of leptin, adiponectin, resistin and PPAR-γ was recorded in the absence and presence of chitosan, O-CMCs and N-CQCs. The experimental results proved that chitosan, O-CMCs and N-CQCs not only lowered the level of plasma leptin, glucose, insulin and total cholesterol in vivo, but down-regulated mRNA expression of leptin and resistin, and up-regulated mRNA expression of adiponectin and PPAR-γ in vitro, to achieve the desired insulin resistance therapy.

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Section: Cytotoxicity Analysissupporting

confidence: 90%

Therapeutic Effect of Carboxymethylated and Quanternized Chitosan on Insulin Resistance in High-Fat-Diet-Induced Rats and 3T3-L1 Adipocytes

Liu

Yang

Song

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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“…[27] The wide scope of CMC-based matrices for biotechnological applications also encompasses antigen delivery systems [28] and nonviral vectors for gene delivery (a more detailed survey is presented in Table S1 in the Supporting Information). [29] Recently, the first in vivo biodegradation studies on FITC-labelled CMC underscored its high application potential. [30] Whereas the productive interaction of chitosan with oxide nanomaterials has been intensely investigated in, for example, catalysis, [31] fuel cell [32] and antibacterial research, [33] the cellular uptake mechanisms of POM/chitosan composites still remain to be explored.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Geisberger

Paulus

Carraro

et al. 2011

Chemistry A European J

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Chitosan and its derivates continue to attract considerable research interest as effective drug carriers with good biocompatibility and high cellular uptake rates. We used these versatile features to tap the considerable biomedical potential of polyoxometalates (POMs) through their encapsulation into a carboxymethyl chitosan (CMC) matrix. The nanocapsules were prepared by ionic gelification with Ca(2+); their size distribution ranges from 60 to 150 nm. Because [Co(4)(H(2)O)(2)(PW(9)O(34))(2)](10-) is well known for its manifold properties, such as antiviral activity, it was selected as a model POM. The resulting composites were characterised with a wide range of analytical methods, which pointed to quantitative encapsulation of intact POMs within the CMC matrix. We studied the biocompatibility of the POM/CMC nanocomposites on HeLa cells through MTT and proliferation assays. Even after prolonged incubation times at high concentrations, the composites did not display cytotoxicity, thereby drastically reducing the side effects of the pristine POMs. This opens up new avenues for designing novel inorganic drug prototypes from bioactive POMs.

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“…The results indicated that electrostatic interactions are the main driving forces in acidic and basic conditions while hydrophobic interactions are the dominating forces in case of neutral conditions and hence OCMchitosan have good potential for increasing the effectiveness of OCM-chitosan for gene therapy (Zhu, Fang, Chan-Park, & Chan, 2009). With the aim of improving the transfection efficiency of, OCM-chitosan-organosilica hybrid nanoparticles were synthesized through a rapid one-step aqueous synthetic approach for gene delivery (Zhang et al, 2009). The hybrid nanoparticles prepared El -Sharif andHussain, 2011 (2011) Five chemically modified chitosans (i) 5-methylpyrrolidinone chitosan (ii) N-carboxymethyl chitosan (iii) N-dicarboxymethyl chitosan (iv) N-dimethylaminopropyl chitosan (v) N-phosphonomethyl chitosan 5-Methylpyrrolidinone chitosan, N-carboxymethyl chitosan and N-phosphonomethyl chitosan exerted effective fungistatic action against Saprolegina parasitica Muzzarelli, Muzzarelli, Tarsi, Miliani, and Cartolari (2001) Schiff bases of CM-chitosan Fusarium oxysporium f. sp.…”

Section: Carboxymethyl Chitosan In Gene Therapymentioning

confidence: 99%

Biomedical applications of carboxymethyl chitosans

Upadhyaya

Singh²,

Agarwal

et al. 2013

Carbohydrate Polymers

298

130

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O-Carboxymethyl-chitosan/organosilica hybrid nanoparticles as non-viral vectors for gene delivery

Cited by 31 publications

References 36 publications

Therapeutic Effect of Carboxymethylated and Quanternized Chitosan on Insulin Resistance in High-Fat-Diet-Induced Rats and 3T3-L1 Adipocytes

Therapeutic Effect of Carboxymethylated and Quanternized Chitosan on Insulin Resistance in High-Fat-Diet-Induced Rats and 3T3-L1 Adipocytes

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Biomedical applications of carboxymethyl chitosans

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