O-glycosylation in plant and mammal cells: the use of chemical inhibitors to understand the biosynthesis and function of O-glycosylated proteins

Mewono, Ludovic; Nguema-Ona, Éric; Gotté, Maxime; Koroney, Abdoul-Salam; Follet-Gueye, Marie-Laure; Driouich, Azeddine; Vicré-Gibouin, Maïté; Aboughe-Angone, Sophie

doi:10.14719/pst.2015.2.2.67

Cited by 6 publications

(1 citation statement)

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“…More than 20 different UDP-GalNAc polypeptide N-acetylgalactosaminyltransferases can be involved in the GalNAc attachment. This GalNAc is further modified by the stepwise attachment of different monosaccharides such as galactose (Gal), GlcNAc, sialic acid and fucose giving rise to diverse mucin-type core O-glycans that play crucial roles in many biological processes (Schjoldager and Clausen, 2012;Mewono et al, 2015).…”

Section: General Features Of Eukaryotic O-glycosylationmentioning

confidence: 99%

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

et al. 2020

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The term microalga refers to various unicellular and photosynthetic organisms representing a polyphyletic group. It gathers numerous species, which can be found in cyanobacteria (i.e., Arthrospira) as well as in distinct eukaryotic groups, such as Chlorophytes (i.e., Chlamydomonas or Chlorella) and Heterokonts (i.e., diatoms). This phylogenetic diversity results in an extraordinary variety of metabolic pathways, offering large possibilities for the production of natural compounds like pigments or lipids that can explain the ever-growing interest of industrials for these organisms since the middle of the last century. More recently, several species have received particular attention as biofactories for the production of recombinant proteins. Indeed, microalgae are easy to grow, safe and cheap making them attractive alternatives as heterologous expression systems. In this last scope of applications, the glycosylation capacity of these organisms must be considered as this post-translational modification of proteins impacts their structural and biological features. Although these mechanisms are well known in various Eukaryotes like mammals, plants or insects, only a few studies have been undertaken for the investigation of the protein glycosylation in microalgae. Recently, significant progresses have been made especially regarding protein N-glycosylation, while O-glycosylation remain poorly known. This review aims at summarizing the recent data in order to assess the state-of-the art knowledge in glycosylation processing in microalgae.

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Section: General Features Of Eukaryotic O-glycosylationmentioning

confidence: 99%

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

et al. 2020

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Arabinogalactan Proteins From Baobab and Acacia Seeds Influence Innate Immunity of Human Keratinocytes In Vitro

Zahid

Despres

Bénard

et al. 2017

Journal Cellular Physiology

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Plant derived arabinogalactan proteins (AGP) were repeatedly confirmed as immunologically as well as dermatologically active compounds. However, little is currently known regarding their potential activity toward skin innate immunity. Here, we extracted and purified AGP from acacia (Acacia senegal) and baobab (Adansonia digitata) seeds to investigate their biological effects on the HaCaT keratinocyte cell line in an in vitro system. While AGP from both sources did not exhibit any cytotoxic effect, AGP from acacia seeds enhanced cell viability. Moreover, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that AGP extracted from both species induced a substantial overexpression of hBD-2, TLR-5, and IL1-α genes. These data suggest that plant AGP, already known to control plant defensive processes, could also modulate skin innate immune responses. J. Cell. Physiol. 232: 2558-2568, 2017. © 2016 Wiley Periodicals, Inc.

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