O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa

Azimi, Sheyda; Thomas, Jacob; Cleland, Sara E.; Curtis, Jennifer E.; Goldberg, Joanna B.; Diggle, Stephen P.

doi:10.1128/mbio.00860-21

Cited by 37 publications

(28 citation statements)

References 70 publications

(107 reference statements)

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“…The results suggest that reducing the production of cell-surface-associated lipopolysaccharides and exopolysaccharides can increase the hydrophobicity of the cell surface (i.e., the interfacial energy among oil, water, and cell surfaces), which can help the cells to be trapped at the oil–water interface. This agrees with the result of an earlier study where Δ wbpL was shown to have an increased relative cell surface hydrophobicity . Based on a similar mechanism, a Staphylococcus sp.…”

Section: Resultssupporting

confidence: 91%

“…We cultured gentamicin-resistant colonies in LB broth devoid of NaCl (LBNS) without antibiotics and plated the colonies on LBNS agar plates containing 10% sucrose. We then selected sucrose-resistant colonies, screened them for gentamicin sensitivity to ensure the loss of the PEX18GM construct, and assessed them for the desired gene deletion by colony PCR and Sanger sequencing of the PCR product …”

Section: Methodsmentioning

confidence: 99%

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Factors Influencing Pseudomonas aeruginosa Initial Adhesion and Evolution at the Dodecane–Water Interface

Zhang,

Zong

et al. 2023

Langmuir

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Bacterial adhesion and evolution at the oil–water interface are important for a broad range of applications such as food manufacturing and microbial-enhanced oil recovery, etc. However, our understanding on bacterial interfacial adhesion and evolution, particularly at the single-cell level, is still far from complete. In this work, by employing Pseudomonas aeruginosa PAO1 at the dodecane–water interface as a model system, we have studied the effects of different factors on bacterial interfacial adhesion and the dynamic evolution of bacterial interfacial behavior at the single-cell level. The results show that PAO1 cells displayed a chemotactic behavior toward dodecane. Among the tested factors, bacterial initial interfacial attachment showed a negative correlation with the secreted cell-surface associated lipopolysaccharide and Psl while a positive correlation with type IV pili. Adding nonbiological surfactant Pluronic F-127, as expected, greatly reduced the cell interfacial adhesion. More importantly, the dynamics analysis of cell attachment/detachment at the dodecane–water interface over a long-time scale revealed a reversible to irreversible attachment transition of cells. This transition is accompanied with the interface aging resulting from bacterial activities, which led to an increase of the interfacial viscoelasticity with time and finally the formation of the gel-like interface. Further analysis demonstrated the important role of exopolysaccharides in the latter process. Our findings provide more details of bacterial oil–water interfacial behavior at the single-cell level and may shed light on developing new strategies for controlling bacterial colonization at the oil–water interface.

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Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Factors Influencing Pseudomonas aeruginosa Initial Adhesion and Evolution at the Dodecane–Water Interface

Zhang,

Zong

et al. 2023

Langmuir

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“…Clinical isolates with different biological characteristics could affect depletion-mediated bacteria-bacteria interactions. For example, recent work demonstrates that LPS O-antigen modifications in P. aeruginosa change cell surface hydrophobicity, which may disrupt the tightly-packed and ordered cell arrangements characteristic of depletion aggregates ( Azimi et al., 2021 ). Other surface modifications that affect surface charge could also affect depletion-mediated bacteria-bacteria or bacteria-polymer interactions.…”

Section: Discussionmentioning

confidence: 99%

The Depletion Mechanism Actuates Bacterial Aggregation by Exopolysaccharides and Determines Species Distribution & Composition in Bacterial Aggregates

Secor

Michaels

Bublitz

et al. 2022

Front. Cell. Infect. Microbiol.

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Bacteria in natural environments and infections are often found in cell aggregates suspended in polymer-rich solutions, and aggregation can promote bacterial survival and stress resistance. One aggregation mechanism, called depletion aggregation, is driven by physical forces between bacteria and high concentrations of polymers in the environment rather than bacterial activity per se. As such, bacteria aggregated by the depletion mechanism will disperse when polymer concentrations fall unless other adhesion mechanisms supervene. Here we investigated whether the depletion mechanism can actuate the aggregating effects of Pseudomonas aeruginosa exopolysaccharides for suspended (i.e. not surface attached) bacteria, and how depletion affects bacterial inter-species interactions. We found that cells overexpressing the exopolysaccharides Pel and Psl remained aggregated after short periods of depletion aggregation whereas wild-type and mucoid P. aeruginosa did not. In co-culture, depletion aggregation had contrasting effects on P. aeruginosa’s interactions with coccus- and rod-shaped bacteria. Depletion caused S. aureus (cocci) and P. aeruginosa (rods) to segregate from each other and S. aureus to resist secreted P. aeruginosa antimicrobial factors resulting in species co-existence. In contrast, depletion aggregation caused P. aeruginosa and Burkholderia sp. (both rods) to intermix, enhancing type VI secretion inhibition of Burkholderia by P. aeruginosa, leading to P. aeruginosa dominance. These results show that in addition to being a primary cause of aggregation in polymer-rich suspensions, physical forces inherent to the depletion mechanism can promote aggregation by some self-produced exopolysaccharides and determine species distribution and composition of bacterial communities.

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“…The second step of biofilm establishment i.e., the micro-colony formation is characterized by bacterial adhesion, aggregation and persistence; each of these phenomena are dependent on cell surface hydrophobicity (CSH). Generally, P. aeruginosa exhibits hydrophobic cell surface, which is correlated with slow growth, strong biofilm formation and persistence (49); clinical isolates of mucoid, slime-producing strains of P. aeruginosa become non-adherent by alteration of CSH, while non-mucoid, non-slime producing strain become adherent with high affinity by increasing CSH (50). Eugenol is shown to inhibit biofilm formation and adhesion on Hep-2 (human larynx epidermoid carcinoma) cell line and on polystyrene by decreasing of CSH in Candida dubiliniensis and C. tropicalis (51).…”

Section: Discussionmentioning

confidence: 99%

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

Basu¹,

Ghosh²,

Sett³

et al. 2022

Preprint

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This study dealt with nanonization of eugenol, a major phytochemical present in basil leaf, which has pharmacological potential as an anti-bacterial agent. Eugenol nanoparticle (ENP) was synthesized by simple ultrasonic cavitation method through emulsification of hydrophobic eugenol into hydrophilic gelatin. Thus, the nanonization process made the water-insoluble eugenol to water-soluble nano-eugenol, making the nano-form bioavailable. The average size of the ENPs was 20-30 nm. Entrapment efficiency of eugenol within gelatin cap was about 80% of the eugenol, that was used as precursor in the nanonization reaction. In vitro release of eugenol from gelatin cap was slow and sustained over a period of five days. The ENP had higher anti-biofilm potency than eugenol for both formation and eradication of biofilm, formed by clinically relevant pathogen Pseudomonas aeruginosa. Minimal biofilm inhibitory concentration and minimal biofilm eradication concentration of ENPs were 2.0 and 4.0 mM respectively. In addition, the measurement of P. aeruginosa biofilm biomass, biofilm pellicle formation, biofilm thickness, amount of biofilm-forming extra-polymeric substance, cell surface hydrophobicity, cell swarming and twitching efficiencies, cellular morphology and biofilm formation in catheter demonstrated that the anti-biofilm efficacy of nano-eugenol was 30-40% higher than that of bulk eugenol. Thus, ENP can be used as a potential drug against pneumonia, a chronic infection in lung caused by P. aeruginosa, which is difficult to treat with antibiotics, due to natural intrinsic resistance of biofilm-formed cells to most antibiotics. The overall actions of ENP have been presented in the figure 1.

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O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa

Cited by 37 publications

References 70 publications

Factors Influencing Pseudomonas aeruginosa Initial Adhesion and Evolution at the Dodecane–Water Interface

Factors Influencing Pseudomonas aeruginosa Initial Adhesion and Evolution at the Dodecane–Water Interface

The Depletion Mechanism Actuates Bacterial Aggregation by Exopolysaccharides and Determines Species Distribution & Composition in Bacterial Aggregates

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

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