1993
DOI: 10.1016/s0021-9258(18)82335-3
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O6-methylguanine residues elicit DNA repair synthesis by human cell extracts

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Cited by 62 publications
(30 citation statements)
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“…Interestingly, we detected robust, MMR-dependent, UDS upon incubation of MNU-treated plasmids in NPE. This observation reveals that, not only are O 6 mG:C lesions recognized by MutSα as previously noted ( Duckett et al, 1999 ; Karran et al, 1993 ), but also the whole MMR repair process is engaged and proceeds to completion. We would also like to stress the high sensitivity of the pull-down assay with respect to MMR protein capture.…”
Section: Discussionsupporting
confidence: 76%
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“…Interestingly, we detected robust, MMR-dependent, UDS upon incubation of MNU-treated plasmids in NPE. This observation reveals that, not only are O 6 mG:C lesions recognized by MutSα as previously noted ( Duckett et al, 1999 ; Karran et al, 1993 ), but also the whole MMR repair process is engaged and proceeds to completion. We would also like to stress the high sensitivity of the pull-down assay with respect to MMR protein capture.…”
Section: Discussionsupporting
confidence: 76%
“…Response of cells to SN1 methylating agents was shown to be initiated at O 6 mG:T mispairs that form upon DNA replication of O 6 mG-containing DNA template and shown to involve the MMR machinery ( Goldmacher et al, 1986 ; Day et al, 1980 ; Karran et al, 1993 ; Yarosh et al, 1983 ). The O 6 mG:T mispair is efficiently recognized by MutSα, the key MMR initiator protein.…”
Section: Discussionmentioning
confidence: 99%
“…1a and [3]). And third, the aberrant DNA synthesis does not occur to a significant extent on a methyl methanesulfonatetreated substrate that contains essentially no O 6 -meG [1].…”
Section: Resultsmentioning
confidence: 99%
“…HeLa cell extracts perform non-replicative DNA synthesis on plasmids methylated with N-methyl-N-nitrosourea (MNU) and containing approximately one O 6 -meG per molecule [1]. Three lines of evidence indicate that this DNA synthesis represents processing of O 6 -meG.…”
Section: Resultsmentioning
confidence: 99%
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