OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds

Abstract

Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs.

Peptides from Animal Origin: A Systematic Review on Biological Sources and Effects on Skin Wounds

“…Currently, identifying animal peptides with healing properties opens a new perspective for the treatment of skin wounds [ 16 , 32 , 34 ]. In general, reviewed studies indicate that peptides originating from mammals, amphibians, fishes, jellyfish, mollusk, and insect exert beneficial effects in stimulating wound closure.…”

“…Due to its antimicrobial, immunomodulatory, promitotic, colagenogenic, and neoangiogenic potential, animal peptides are suggested as promising agents for new therapeutic approaches in skin wound treatment [ 1 , 12 , 15 , 16 ]. Besides, their molecular abundance, low cost of isolation techniques, high molecular stability, and their broad spectrum of biological properties are also encouraging characteristics.…”

Peptides for Skin Protection and Healing in Amphibians

“…The OA-GL21 (Table 1) peptide isolated from by Odorrana andersonii exerts the ability to promote the WH of human keratinocytes and human fibroblasts in a dose- and time-dependent manner, mainly by promoting cell migration rather than inducing cell proliferation [34].…”

“…In a mouse model with deep skin wounds, Cathelicidin-OA1 accelerates re-epithelialization and granulation tissue formation by enhancing the recruitment of macrophages to the wound site, with effects comparable to those exerted by epidermal growth factor (EGF). TNF-α, a pro-inflammatory cytokine, can be upregulated by peptides in the early phase of inflammation during WH, thus becoming responsible for recruiting inflammatory cells to the wound site and exerting predominant chemotactic functions [34].…”

“…The activities of amphibian skin-derived peptides are often overlapping, thus, it is of note that peptides indicated to exert a strong WH power also present weak antioxidant activities, for example, OA-GL21 [34], OM-LV20 [35], and Cathelicidin-OA1 [49] (Table 2).…”