Soil moisture is the main limiting factor for crop growth and the sustainable development of oases in arid desert areas. Therefore, the temporal and spatial variation and infiltration laws of oasis soil moisture should be studied. The objective of this study is to reveal the influencing factors of the spatial-temporal variation of layered soil and sediment moisture and infiltration characteristics under irrigation in desert oases. Hydraulic conductivities were measured using the double-ring infiltrometer, while the regional and site soil moistures were measured and calibrated using weighted method and neutron moisture meter. Deterministic spatial interpolation methods, including multiquadric radial basis function, inverse distance weighted, and local polynomial regression isogram, were adopted to map the regional distribution of hydraulic conductivities, spatial soil moistures, and spatial-temporal isogram of the point site soil moistures in Yaoba Oasis, respectively. Results showed that the leading influencing factors of the (1) regional spatial soil moisture were soil and sediment permeability, stream link direction, microclimate, and dewfalls; (2) spatial layered soil and sediment moistures were microclimate and dew condensation; and (3) spatial-temporal variation at the point site profiles were soil texture, water requirement, and preferential flow. Under irrigation, soil moisture increased significantly, in which the maximum increase was 10.8 times the original state, while the recharging depth substantially increased up to 580 cm with the preferential flow. The spatial-temporal variation of the soil moisture under irrigation indicated that the best irrigation frequency should be 15 days per time. Moreover, the infiltration process can be divided into the preferential flow, piston flow, and balanced infiltration stages.