Obesity does not affect the healing of femur fractures in mice

Histing, Tina; Andonyan, A.; Klein, Moritz; Scheuer, Cláudia; Stenger, David; Holstein, Joerg H.; Veith, Nils T.; Pohlemann, Tim; Menger, Michael D.

doi:10.1016/j.injury.2016.04.030

Cited by 20 publications

(19 citation statements)

References 72 publications

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“…Explanted wt and Trpv6 mt/mt embryos as well as femurs from 9 and 24 weeks were explanted and scanned using a Quantum Fx μCT system for embryos or a high‐resolution micro‐CT system (SkyScan 1172; Bruker, Kontich, Belgium) for adult femurs. Biomechanical analysis was performed using a three‐point bending device (Mini Zwick 22.5; Zwick GmbH, Germany) and we performed a destructive and a nondestructive three‐point bending test to measure the stiffness of the femurs …”

Section: Methodsmentioning

confidence: 99%

“…Biomechanical analysis was performed using a three-point bending device (Mini Zwick 22.5; Zwick GmbH, Germany) and we performed a destructive and a nondestructive three-point bending test to measure the stiffness of the femurs. (27) Isolation of primary mouse placenta trophoblast cells and 45 Ca 2þ uptake Placentas were isolated according to Pennington and colleagues. (28) Primary trophoblast cells from wt and homozygous Trpv6 -/placentas at E14.5 were seeded at 100,000 to 200,000 cells per 2.5-cm dish to measure 45 Ca 2 þ uptake.…”

Section: Transcriptome Analysismentioning

confidence: 99%

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Maternal Transient Receptor Potential Vanilloid 6 (Trpv6) Is Involved In Offspring Bone Development

Fecher‐Trost

Lux

Busch

et al. 2019

Journal of Bone and Mineral Research

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Embryonic growth and bone development depend on placental Ca2+ transport across the feto‐maternal barrier to supply minerals to the fetus. The individual factors and cellular mechanisms that regulate placental Ca2+ transfer, however, are only beginning to emerge. We find that the Ca2+‐selective transient receptor potential vanilloid 6 (TRPV6) channel is expressed in trophoblasts of the fetal labyrinth, in the yolk sac, and in the maternal part of the placenta. Lack of functional TRPV6 channels in the mother leads to a reduced Ca2+ content in both placenta and embryo. Ca2+ uptake in trophoblasts is impaired in the absence of Trpv6. Trpv6‐deficient embryos are smaller, have a lower body weight, and shorter and less calcified femurs. The altered cortical bone microarchitecture persists in adulthood. We show that TRPV6's Ca2+‐conducting property causes this embryonic and bone phenotype. Our results show that TRPV6 is necessary for the Ca2+ uptake in trophoblasts and that TRPV6 deficiency in the placenta leads to reduced embryo growth, minor bone calcification, and impaired bone development. © 2019 American Society for Bone and Mineral Research.

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Section: Methodsmentioning

confidence: 99%

Section: Transcriptome Analysismentioning

confidence: 99%

Maternal Transient Receptor Potential Vanilloid 6 (Trpv6) Is Involved In Offspring Bone Development

Fecher‐Trost

Lux

Busch

et al. 2019

Journal of Bone and Mineral Research

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“…A working gauge length of 6 mm was used. Applying a gradually increasing bending force with 1 mm/min, the bending stiffness (N/mm) was calculated from the linear elastic part of the load displacement diagram 56 . The application of only a non-destructive force was controlled macroscopically and, later on, microscopically during the histological analysis.…”

Section: Radiological Analysismentioning

confidence: 99%

“…At a magnification of × 1.25 (BX60, Olympus, Tokyo, Japan; Axio Cam and Axio Vision 3.1, Carl Zeiss, Oberkochen, Germany) structural indices were calculated according to the suggestion provided by Gerstenfeld et al 60 using the ImageJ Analysis System (NIH, Bethesda, MD, USA). These included total callus area (bone, cartilaginous and fibrous callus area)/femoral bone diameter (cortical width plus marrow diameter) at the fracture gap (CAr/BDm [mm]), bone (total osseous tissue) callus area/total callus area (TOTAr/CAr [%]), cartilaginous callus area/total callus area (CgAr/CAr [%]), and fibrous tissue callus area/total callus area (FTAr/ CAr [%]) 18,56 . Additionally, tartrate-resistant acid phosphatase (TRAP) activity was analyzed in the callus at 2 and 5 weeks after fracture healing.…”

Section: Radiological Analysismentioning

confidence: 99%

Pantoprazole impairs fracture healing in aged mice

Menger

Bremer

Scheuer

et al. 2020

Sci Rep

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Proton pump inhibitors (PPIs) belong to the most common medication in geriatric medicine. They are known to reduce osteoclast activity and to delay fracture healing in young adult mice. Because differentiation and proliferation in fracture healing as well as pharmacologic actions of drugs markedly differ in the elderly compared to the young, we herein studied the effect of the PPI pantoprazole on bone healing in aged mice using a murine fracture model. Bone healing was analyzed by biomechanical, histomorphometric, radiological and protein biochemical analyses. The biomechanical analysis revealed a significantly reduced bending stiffness in pantoprazole-treated animals when compared to controls. This was associated with a decreased amount of bone tissue within the callus, a reduced trabecular thickness and a higher amount of fibrous tissue. Furthermore, the number of osteoclasts in pantoprazole-treated animals was significantly increased at 2 weeks and decreased at 5 weeks after fracture, indicating an acceleration of bone turnover. Western blot analysis showed a lower expression of the bone morphogenetic protein-4 (BMP-4), whereas the expression of the pro-angiogenic parameters was higher when compared to controls. Thus, pantoprazole impairs fracture healing in aged mice by affecting angiogenic and osteogenic growth factor expression, osteoclast activity and bone formation.

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“…Mice were starved for 6 h prior to sacrifice and anesthetized by intraperitoneal injection of ketamine (75 mg/kg) and rompun (xylazine) (15 mg/kg) (20). To measure the sign of consciousness, we used a physical stimulus method, toes were pinched with atraumatic forceps and the reflex was observed.…”

Section: Determination Of Abdominal Fat Volume By Micro-computed Tomomentioning

confidence: 99%

Chrysanthemumï¿½indicum L. ethanol extract reduces high‑fat diet‑induced obesity in mice

et al. 2018

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The present study was undertaken to investigate the mechanism behind the anti-obesity effect of the 50% ethanol extract of L. flowers (CIEE) in a mouse model of high-fat diet (HFD)-induced obesity. Male C57BL/6J mice (six mice in each group) were administered CIEE (8, 40 and 200 mg/kg) for 6 weeks while being fed with a HFD. (GC) was used as the positive control and was administered in the same manner as CIEE. Results demonstrated that oral administration of CIEE significantly reduced body weight, epididymal white adipose tissue (EWAT), liver weight and serum levels of total cholesterol and triglyceride (P<0.05). In addition, CIEE reduced serum leptin and increased adiponectin levels. CIEE significantly downregulated peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein-α and fatty acid synthase expression levels in EWAT, and upregulated the protein expression of PPARα in liver tissue of HFD-fed obese mice (P<0.05). These results suggested that L. flowers may be a potentially effective therapeutic agent for obesity and its associated complications.

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Obesity does not affect the healing of femur fractures in mice

Cited by 20 publications

References 72 publications

Maternal Transient Receptor Potential Vanilloid 6 (Trpv6) Is Involved In Offspring Bone Development

Maternal Transient Receptor Potential Vanilloid 6 (Trpv6) Is Involved In Offspring Bone Development

Pantoprazole impairs fracture healing in aged mice

Chrysanthemumï¿½indicum L. ethanol extract reduces high‑fat diet‑induced obesity in mice

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