Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry

Kim, Min Jung; Yang, Hye Jeong; Kim, Jin Hee; Ahn, Chang Won; Lee, Jong Ho; Kim, Kang Sung; Kwon, Dae Young

doi:10.1155/2013/874981

Cited by 44 publications

(44 citation statements)

References 32 publications

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“…In the present study, the principal metabolites identified reflecting abnormal lipid metabolism were different from those identified in previous metabonomics studies of highfat diet-induced obese mice (Kim et al, 2011) and of obese individuals receiving black soybean peptide in a weight-lowering trial (Kim et al, 2013). In the present study, the principal metabolites identified reflecting abnormal lipid metabolism were different from those identified in previous metabonomics studies of highfat diet-induced obese mice (Kim et al, 2011) and of obese individuals receiving black soybean peptide in a weight-lowering trial (Kim et al, 2013).…”

Section: Principal Changes In Metabolites Of Obesity With Hyperlipemiacontrasting

confidence: 99%

Metabolomic analysis of serum from obese adults with hyperlipemia by UHPLC‐Q‐TOF MS/MS

Wang

Liu

et al. 2015

Biomedical Chromatography

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The prevalence of obesity has dramatically increased and poses a major threat to human health. Obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases. Differences in metabolomic profiling of serum between obese (with hyperlipemia) and normal-weight men (n = 30 in each group) were investigated using ultrahigh-pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF MS/MS) and partial least-squares-discriminant analysis (PLS-DA). Obese men showed higher levels of weight, body mass index, fat mass, systolic blood pressure, fasting plasma glucose, triglyeride, total cholesterol, insulin, HOMA-IR and high-sensitivity CRP. Obese and normal-weight groups were clearly discriminated from each other on a PLS-DA score plot and nine major metabolites contributing to the discrimination were assigned, including increased 2-octenoylcarnitine, eicosadienoic acid, 12-hydroperoxyeicosatetraenoic acid, 4-hydroxyestrone sulfate, lysoPE[18:1(11Z)/0:0], thromboxane B2 and pyridinoline and decreased vitamin D3 glucosiduronate and 9,10-DHOME. These metabolites were associated with lipid metabolism and obesity-related diseases, and reflected the metabolic differences between normal and obese men, which may be important for future clinical diagnosis, treatment and assessment of the therapeutic effect on obesity-related chronic disease.

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Section: Principal Changes In Metabolites Of Obesity With Hyperlipemiacontrasting

confidence: 99%

Metabolomic analysis of serum from obese adults with hyperlipemia by UHPLC‐Q‐TOF MS/MS

Wang

Liu

et al. 2015

Biomedical Chromatography

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“…Interestingly deregulated lipids often contain linoleic acid 18:2. LPC(18:2) is a well-known negative correlate of insulin resistance, dysglycemia and diabetes (Ferrannini et al, 2013; Floegel et al, 2013; Gall et al, 2010; Wang-Sattler et al, 2012) and LPCs have been shown to be reduced in obesity and T2D (Kim et al, 2011; Kim et al, 2013; Wang-Sattler et al, 2012). The global decrease of phospholipids may be due to a reduced biosynthesis because phospholipid precursors choline, phosphorylcholine and sn-glycero-3-phosphoethanolamine were also found in lower quantities in the plasma of Nat1 ko mice.…”

Section: Discussionmentioning

confidence: 99%

Nat1 Deficiency Is Associated with Mitochondrial Dysfunction and Exercise Intolerance in Mice

et al. 2016

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Summary We recently identified human N-acetyltransferase 2 (NAT2) as an insulin resistance (IR) gene. Here we examine the cellular mechanism linking NAT2 to IR and find that Nat1 (mouse ortholog of NAT2) is co-regulated with key regulators of mitochondria. RNA-interference mediated silencing of Nat1 led to mitochondrial dysfunction characterized by increased intracellular reactive oxygen species and mitochondrial fragmentation as well as decreased mitochondrial membrane potential, biogenesis, mass, cellular respiration and ATP generation. These effects were consistent in 3T3-L1 adipocytes, C2C12 myoblasts and in tissues from Nat1 deficient mice including white adipose tissue, heart and skeletal muscle. Nat1 deficient mice had changes in plasma metabolites and lipids consistent with a decreased ability to utilize fats for energy and a decrease in basal metabolic rate and exercise capacity without altered thermogenesis. Collectively, our results suggest that Nat1 deficiency results in mitochondrial dysfunction, which may constitute a mechanistic link between this gene and IR.

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“…Highly responsive to energy restriction had reductions in adiposity, and distinguishable blood metabolite signature, including reduction in plasma free fatty acids stearic acid (18:0) and palmitic acid (16:0) [56]. Notably, the lysoPCs incorporating these fatty acids were likewise decreased in the weight-loss trial conducted with the black soybean intervention, therefore most likely reflecting the metabolic impact of the weight loss in general [40].…”

Section: Dietary Interventions With Focus On Weight Managementmentioning

confidence: 97%

“…Another dietary intervention with a specific protein source was conducted with black soybean peptides for 12 weeks to induce weight loss [40]. The test group had reduced BMI accompanied by numerous differential features as assessed by PLS-DA from the LC-qTOF-MS analysis.…”

Section: Dietary Interventions With Focus On Weight Managementmentioning

confidence: 99%

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Application of Metabolomics to Assess Effects of Controlled Dietary Interventions

Hanhineva

2015

Curr Nutr Rep

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The utilization of metabolomics technologies as part of nutritional investigations has rapidly increased during the last few years. The application of both nuclear magnetic resonance (NMR) and mass spectrometry (MS) based technologies is providing wide information on both the dietderived compounds and their metabolites related to specific foods or diets, as well as aiding the in-depth exploration of the endogenous metabolic phenomena related to different diets. The analytical accuracy nowadays enables examination of metabolite composition of both urine and plasma to very high molecular detail even at low concentrations, and will be focal in gaining wider metabolic understanding of the relationship between eating habits and maintenance of good health.

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Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry

Cited by 44 publications

References 32 publications

Metabolomic analysis of serum from obese adults with hyperlipemia by UHPLC‐Q‐TOF MS/MS

Metabolomic analysis of serum from obese adults with hyperlipemia by UHPLC‐Q‐TOF MS/MS

Nat1 Deficiency Is Associated with Mitochondrial Dysfunction and Exercise Intolerance in Mice

Application of Metabolomics to Assess Effects of Controlled Dietary Interventions

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