BackgroundMicrodermabrasion is a cosmetic procedure that has gained popularity for skin rejuvenation by causing repetitive intraepidermal injury to stimulate the proliferation of fibroblasts and collagen production. Various clinical studies have demonstrated microdermabrasion's effectiveness in skin rejuvenation; however, most of these studies rely on clinical observation and scoring by observers rather than histologic or microscopic analysis. In our single‐center prospective study, we used line‐field confocal optical coherence tomography (LC‐OCT), to non‐invasively visualize the early effects of one microdermabrasion treatment on the facial epidermal and dermal structure.AimUsing LC‐OCT, this study aims to elucidate the microscopic and histological effects of microdermabrasion on epidermal and dermal structures, including epidermal thickness, as well as collagen and vascular patterns.Patients/MethodsEight volunteers (Fitzpatrick skin types II–V) underwent one treatment of microdermabrasion. LC‐OCT and VISIA imaging were performed before and 10 min after microdermabrasion, and at 48‐h follow‐up. Subjective evaluations of skin texture and adverse reactions were assessed 1 week posttreatment via a telephone call.ResultsCompared to LC‐OCT images before treatment, images captured after one treatment of microdermabrasion showed a decrease in thickness and number of undulations in the stratum corneum. In the superficial dermis, enhancement in fibrillar collagen, as demonstrated by an increased prominence of crisscrossing hyper‐refractile strands, was visualized. This was consistent with subjective and objective improvement in facial rhytids calculated by VISIA skin analysis.ConclusionsTreatment monitoring with LC‐OCT demonstrated consistent histopathological changes with clinical visual improvement. Therefore, LC‐OCT, has the potential to enable long‐term histopathological monitoring of microdermabrasion and other cosmetic procedures without biopsy.