Obligate Gut Symbiotic Association with Caballeronia in the Mulberry Seed Bug Paradieuches dissimilis (Lygaeoidea: Rhyparochromidae)

Ishigami, K.; Jang, Seonghan; Itoh, Hideomi; Kikuchi, Yoshitomo

doi:10.1007/s00248-022-02117-2

Cited by 8 publications

(2 citation statements)

References 92 publications

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“…Members of the superfamily Coreoidae harbor Caballeronia symbionts in midgut crypts organized in two rows ( Ohbayashi et al, 2019b ; Ishigami et al, 2021 ; Ohbayashi et al, 2022 ), as shown in the bean bug. Members of the superfamily Lygaeoidea carry Caballeronia symbionts in elongated tubular-form midgut crypts ( Kikuchi et al, 2011a ; Ishigami et al, 2022 ). Despite this morphological diversity, considering that the developmental site of crypts is common to the posterior region of the midgut (i.e.…”

Section: Discussionmentioning

confidence: 99%

Symbiont coordinates stem cell proliferation, apoptosis, and morphogenesis of gut symbiotic organ in the stinkbug-Caballeronia symbiosis

et al. 2023

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The bean bug Riptortus pedestris obtains a specific bacterial symbiont, Caballeronia insecticola (Burkholderia insecticola), from the environmental soil and harbors it in the posterior midgut region that is composed of hundreds of crypts. While newly hatched aposymbiotic insects possess primordial midgut crypts with little or no lumen, colonization of C. insecticola triggers swift development of the symbiotic organ, forming enlarged and opened crypts, and the symbiont subsequently fills the luminal cavities of those mature crypts. The cellular processes of crypt development triggered by C. insecticola colonization are poorly understood. Here we identified a fundamental mechanism of the symbiont-mediated midgut development by investigating cell cycles of intestinal epithelial cells. Intestinal stem cells of the bean bug are located and proliferate at the crypt base. Differentiated enterocytes migrate upward along the epithelial cell layer of the crypt as the midgut develops, induction of apoptosis in enterocytes primarily occurred on the tip side of the crypts, and apoptotic cells then eventually were shed from the crypts into the hemolymph. The proliferation rate of the stem cells at the base of the crypts was low while a high apoptotic rate was observed at the crypt tip in aposymbiotic insects, resulting in undeveloped short crypts. On the contrary, the gut-colonizing C. insecticola promoted the proliferation of the stem cells at the base of crypts and simultaneously inhibited apoptosis at the tip of crypts, resulting in a net growth of the crypts and the generation of a crypt lumen that becomes colonized by the bacterial symbiont. These results demonstrated that the Caballeronia symbiont colonization induces the development of the midgut crypts via finely regulating the enterocyte cell cycles, enabling it to stably and abundantly colonize the generated spacious crypts of the bean bug host.

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Section: Discussionmentioning

confidence: 99%

Symbiont coordinates stem cell proliferation, apoptosis, and morphogenesis of gut symbiotic organ in the stinkbug-Caballeronia symbiosis

et al. 2023

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“…Most members of the stink bug superfamilies Coreoidea, Lygaeoidea, and Pyrrhocoroidea develop crypts at the posterior end of their midgut, which environmentally acquired bacteria, belonging almost exclusively to Burkholderia sensu lato, occupy [3,[10][11][12][13][14]. Midgut crypts of one of such stink bugs, the bordered plant bug Physopelta gutta (superfamily: Pyrrhocoroidea; family: Largidae), are of the tubular type, and densely colonized by symbiotic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Paraburkholderia largidicola sp. nov., a gut symbiont of the bordered plant bug Physopelta gutta

Yashima,

Terata,

Sakamoto

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacteria, designated strains F2T and PGU16, were isolated from the midgut crypts of the bordered plant bug Physopelta gutta, collected in Okinawa prefecture, Japan. Although these strains were derived from different host individuals collected at different times, their 16S rRNA gene sequences were identical and showed the highest similarity to Paraburkholderia caribensis MWAP64T (99.3 %). The genome of strain F2T consisted of two chromosomes and two plasmids, and its size and G+C content were 9.28 Mb and 62.4 mol% respectively; on the other hand, that of strain PGU16 consisted of two chromosomes and three plasmids, and its size and G+C content were 9.47 Mb and 62.4 mol%, respectively. Phylogenetic analyses revealed that these two strains are members of the genus Paraburkholderia. The digital DNA–DNA hybridization value between these two strains was 92.4 %; on the other hand, the values between strain F2T and P. caribensis MWAP64T or phylogenetically closely related Paraburkholderia species were 44.3 % or below 49.1 %. The predominant fatty acids of both strains were C16 : 0, C17 : 0 cyclo, summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c), and C19 : 0 cyclo ω8c, and their respiratory quinone was ubiquinone 8. Based on the above genotypic and phenotypic characteristics, strains F2T and PGU16 represent a novel species of the genus Paraburkholderia for which the name Paraburkholderia largidicola sp. nov. is proposed. The type strain is F2T (=NBRC 115765T=LMG 32765T).

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Effect of seed priming with polyethylene glycol, distilled water, and sorbitol on physical, chemical quality parameters, and nodule microbiota of lentil

Çalık Koç,

Rezaei,

Kahraman Ilıkkan

et al. 2024

Braz J Microbiol

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Obligate Gut Symbiotic Association with Caballeronia in the Mulberry Seed Bug Paradieuches dissimilis (Lygaeoidea: Rhyparochromidae)

Cited by 8 publications

References 92 publications

Symbiont coordinates stem cell proliferation, apoptosis, and morphogenesis of gut symbiotic organ in the stinkbug-Caballeronia symbiosis

Symbiont coordinates stem cell proliferation, apoptosis, and morphogenesis of gut symbiotic organ in the stinkbug-Caballeronia symbiosis

Paraburkholderia largidicola sp. nov., a gut symbiont of the bordered plant bug Physopelta gutta

Effect of seed priming with polyethylene glycol, distilled water, and sorbitol on physical, chemical quality parameters, and nodule microbiota of lentil

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