Obligately and facultatively autotrophic, sulfur- and hydrogen-oxidizing thermophilic bacteria isolated from hot composts

Beffa, Trello; Blanc, Michel; Aragno, Michel

doi:10.1007/s002030050293

Cited by 69 publications

(50 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Members of the Aquificales have been isolated from shallow marine (Huber et al, 1992 ;Nishihara et al, 1990) and terrestrial hydrothermal systems (Kryukov et al, 1983 ;Kawasumi et al, 1984 ;Shima & Suzuki, 1993 ;Huber et al, 1998 ;Kristjansson et al, 1985), from heated compost (Beffa et al, 1996) and, recently, from a deepsea hydrothermal vent (Reysenbach et al, 2000a). On the basis of molecular ecological analyses, the Aquificales appear to be dominant members of communities in near-neutral terrestrial thermal springs in Japan (Yamamoto et al, 1998), Iceland (Hjo$ rleifsdottir et al, 2001) and Yellowstone National…”

Section: Introductionmentioning

confidence: 99%

Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents.

Götz¹,

Banta²,

Beveridge³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

(optimum at 70 SC). Both strains grew fastest at 2 5 % (w/v) NaCl and at pH 6, although growth was observed from pH 4 7 to pH 7 5. EX-H1 T and EX-H2 T were able to use elemental sulfur, thiosulfate or hydrogen as an electron donor, and oxygen (2-3 %, v/v) or nitrate as an electron acceptor. EX-H1 T was also able to use elemental sulfur as an electron acceptor. EX-H1 T and EX-H2T further differed in their genomic GMC content (38 5 and 37 4 mol %, respectively) and 16S rRNA sequences (4 % difference). Maximum-likelihood analysis of the 16S rRNA phylogeny placed both isolates within the Aquificales as a distinct lineage and showed them to be only about 85 % similar to Aquifex pyrophilus. On the basis of phenotypic and phylogenetic characteristics, it is proposed that EX-H1 T and EX-H2 T belong to a new genus within the Aquificales, namely Persephonella gen. nov. It is further proposed that EX-H1 T be named Persephonella marina sp. nov., the type species of the genus, and that EX-H2 T be named Persephonella guaymasensis sp. nov., a second species in this genus.

show abstract

Section: Introductionmentioning

confidence: 99%

Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents.

Götz¹,

Banta²,

Beveridge³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…Cultivation of microorganisms extracted from compost samples allows one to obtain pure cultures which can be used for further taxonomic or physiological characterizations (2,4,11,13,14,16,47). Rapid molecular PCR-based techniques, such as amplified ribosomal DNA restriction analysis (ARDRA), are useful for comparison of a large number of isolates at the phylogenetic level (48).…”

mentioning

confidence: 99%

Succession of Microbial Communities during Hot Composting as Detected by PCR–Single-Strand-Conformation Polymorphism-Based Genetic Profiles of Small-Subunit rRNA Genes

Peters

Koschinsky

Schwieger

et al. 2000

Appl Environ Microbiol

295

181

View full text Add to dashboard Cite

A cultivation-independent technique for genetic profiling of PCR-amplified small-subunit rRNA genes (SSU rDNA) was chosen to characterize the diversity and succession of microbial communities during composting of an organic agricultural substrate. PCR amplifications were performed with DNA directly extracted from compost samples and with primers targeting either (i) the V4-V5 region of eubacterial 16S rRNA genes, (ii) the V3 region in the 16S rRNA genes of actinomycetes, or (iii) the V8-V9 region of fungal 18S rRNA genes. Homologous PCR products were converted to single-stranded DNA molecules by exonuclease digestion and were subsequently electrophoretically separated by their single-strand-conformation polymorphism (SSCP). Genetic profiles obtained by this technique showed a succession and increasing diversity of microbial populations with all primers. A total of 19 single products were isolated from the profiles by PCR reamplification and cloning. DNA sequencing of these molecular isolates showed similarities in the range of 92.3 to 100% to known gram-positive bacteria with a low or high G؉C DNA content and to the SSU rDNA of ␥-Proteobacteria. The amplified 18S rRNA gene sequences were related to the respective gene regions of Candida krusei and Candida tropicalis. Specific molecular isolates could be attributed to different composting stages. The diversity of cultivated bacteria isolated from samples taken at the end of the composting process was low. A total of 290 isolates were related to only 6 different species. Two or three of these species were also detectable in the SSCP community profiles. Our study indicates that community SSCP profiles can be highly useful for the monitoring of bacterial diversity and community successions in a biotechnologically relevant process.

show abstract

“…Although many investigations on the responsible microorganisms in the compo sting process have focused on the temperature pattern, especially at the thermophilic stage (Nakasaki et al 1985;Strom 1985a, b;Beffa et al 1996;Herrmann and Shann 1997;Klamer and BiHith 1998). no studies have been conducted to elucidate the relationship between the changes in the composition of organic constituents of the composting materials and the succession of responsible microbiota during the composting process.…”

mentioning

confidence: 99%

Succession of microbiota estimated by phospholipid fatty acid analysis and changes in organic constituents during the composting process of rice straw

Cahyani

Watanabe

Matsuya

et al. 2002

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

Obligately and facultatively autotrophic, sulfur- and hydrogen-oxidizing thermophilic bacteria isolated from hot composts

Cited by 69 publications

References 35 publications

Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents.

Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents.

Succession of Microbial Communities during Hot Composting as Detected by PCR–Single-Strand-Conformation Polymorphism-Based Genetic Profiles of Small-Subunit rRNA Genes

Succession of microbiota estimated by phospholipid fatty acid analysis and changes in organic constituents during the composting process of rice straw

Contact Info

Product

Resources

About