Objectives
1) To localize quantitatively the major biochemical constituents of native adult human septal cartilage across whole septa.
Study Design
Prospective, basic science
Methods
The nasal septa from seven cadavers were partitioned into 24 separate regions: six from caudal to cephalic and 4 from dorsal to ventral. Biochemical assays were used to determine the quantities, relative to wet weight, of the major constituents of cartilage: chondrocytes, collagen and sulfated glycosaminoglycan.
Results
On average, each milligram of wet cartilage contained 24,900 cells, 73.9 micrograms collagen, and 17.1 micrograms sulfated glycosaminoglycan. Cell number showed no significant variation across the septa. In contrast, the caudal regions of the septa were associated with higher levels of collagen, the ventral regions correlated with higher levels of sulfated glycosaminoglycan, and the dorsal regions were associated with an elevated ratio of collagen to sulfated glycosaminoglycan.
Conclusion
This study represents the first characterization of the biochemical composition of native human septal cartilage across whole septa. Quantities of collagen and sulfated glycosaminoglycan showed region-specific variation across the septum. The localized pattern of collagen and sulfated glycosaminoglycan deposition are consistent with the significance of preserving the “L-strut” during rhinoplasty and other nasal reconstructive procedures. In addition, it may assist in defining design goals for tissue-engineered septal neocartilage constructs to meet specific reconstructive needs in the future.
Level of Evidence
N/A