Obliteration of bacterial growth and biofilm through ROS generation by facilely synthesized green silver nanoparticles

Qayyum, Shariq; Oves, Mohammad; Khan, Asad U.

doi:10.1371/journal.pone.0181363

Cited by 129 publications

(96 citation statements)

References 31 publications

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“…One of the important property of such nanoparticles is antimicrobial activity. Many green silver nanoparticles have been reported for antibacterial and antibiofilm activity through mechanisms like ROS generation (Qayyum et al, 2017). Also supportive studies on plant derived silver nanoparticles conclude that biogenic nanomaterials are biocompatible and an effective therapeutic agent against bacterial, fungal infections, and cancer treatment (Oves et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

100

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Biosynthesis of metallic nanoparticles has acquired particular attention due to its economic feasibility, low toxicity, and simplicity of the process. In this study, extracellular synthesis of silver and zinc nanoparticle was carried out by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Characterization studies revealed synthesis of 40 and 60 nm nanoparticles of silver (AgNP) and zinc (ZnNP), respectively, with distinct morphology as observed in TEM and its crystalline nature confirmed by XRD. DLS, zeta potential, NTA, and FTIR studies further characterized nanoparticles giving data about its size, stability, and functional groups. Considering the toxicity of nanoparticles the evaluation of antimicrobial activity was studied in the range of non-toxic concentration for normal cell lines. Silver nanoparticles were found to be the most effective antimicrobial against all tested strains and drug-resistant clinical isolates of MRSA, VRE, ESBL, MDR, Pseudomonas aeruginosa with MIC in the range of 1.25–5 mg/ml. Zinc nanoparticles were found to be specifically active against Gram-positive bacteria like Staphylococcus aureus including its drug-resistant variant MRSA. Both AgNP and ZnNP were found to be effective against Mycobacterium tuberculosis and its MDR strain with MIC of 1.25 mg/ml. The synergistic action of nanoparticles assessed in combination with a common antibiotic gentamicin (590 μg/mg) used for the treatment of various bacterial infections by Checker board assay. Silver nanoparticles profoundly exhibited synergistic antimicrobial activity against drug-resistant strains of MRSA, ESBL, VRE, and MDR P. aeruginosa while ZnNP were found to give synergism with gentamicin only against MRSA. The MRSA, ESBL, and P. aeruginosa strains exhibited MIC of 2.5 mg/ml except VRE which was 10 mg/ml for both AgNPs and ZnNPs. These results prove the great antimicrobial potential of AgNP and ZnNP against drug-resistant strains of community and hospital-acquired infections and opens a new arena of antimicrobials for treatment, supplementary prophylaxis, and prevention therapy.

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Section: Introductionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

100

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“…[58]. Qayyum et al reported that AgNPs could induce bacterial cells to produce ROS, which might be the cause of cell lysis or distortion of bacterial membranes [59]. Therefore, it is rational to assume that antimicrobial activity of AgNPs can be ascribed to the damage of the cell membrane and the leakage of cytoplasm of Ao strain RS-2.…”

Section: Bacterial Cell Morphologymentioning

confidence: 99%

Biosynthesis of silver nanoparticle from pomelo (Citrus Maxima) and their antibacterial activity against acidovorax oryzae RS-2

Ali

Yao

et al. 2020

Mater. Res. Express

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Silver nanoparticles (AgNPs), synthesized with plant materials, are considered to be an emerging field of agriculture for their eco-friendly and outstanding antibacterial attributes. In this study, we synthesized AgNPs using pomelo (Citrus maxima) fruit extract as a biological capping and reducing material. The particle size was determined as 11.3-12.8 nm by using UV-vis spectrophotometer, TEM and x-ray diffraction analysis. UV-vis spectrophotometer analysis also confirmed the formation of AgNPs in colloidal solution and showed a maximum absorption at 426 nm. Fourier transform infrared spectra was used to analysed the involvement of biological molecule in AgNPs synthesis. The minimum inhibitory concentration of AgNPs against Acidovorax oryzae strain RS-2 was determined as 25 μg ml −1 by agar well diffusion and bacterial growth assay. In addition, bacterial viability and swarming motility were significantly inhibited by AgNPs. Compared with the control, 25 μg ml −1 of AgNPs lower bacterial biofilm formation up to 68.24%. The bacterial cell wall damaged by AgNPs was observed t TEM. Furthermore, AgNPs treatment resulted into the down regulation of expression of many type VI secretion system related genes, suggesting that AgNPs also have an effect on the virulence of bacteria. The overall conclusion of this study suggests that AgNPs can play an important role in controlling A. oryzae.

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“…To improve the antimicrobial efficacy, an increasing number of novel silver materials have been developed . Silver nanoparticles (Ag NPs) are one of the most attractive material for catheter coatings [Fig.…”

Section: Antimicrobial Strategies For Urinary Cathetersmentioning

confidence: 99%

“…To improve the antimicrobial efficacy, an increasing number of novel silver materials have been developed. [38][39][40][41][42][43][44][45][46][47][48] Silver nanoparticles (Ag NPs) are one of the most attractive SCHEME 2. Mechanisms of bactericidal silver ion.…”

Section: Bactericidal Coatingsmentioning

confidence: 99%

“…The Ag NPs are primarily composed of Ag 0 metal with a particle size of <100 nm and very large surfaceto-volume ratio. The microorganisms inhibition mechanism of Ag NPs is related to (1) the release of antibacterial Ag + ion from particle surface [38][39][40][41][42][43][44][45][46][47][48] ; (2) the incorporation of Ag NPs into the bacterial membrane, leading to structural damages and compromised membrane functions, including increase in permeability, disruption of regulated plasma membrane transport, and eventually, causing cell death. 49,50 In addition, silver can be combined with other active materials, showing improved antimicrobial efficacy, for instance, silver coating releasing hydroxyapatite materials, 53 52 The silver-impregnated catheters will continue to play an important role, owing to its broad-spectrum antimicrobial ability, simple preparation, and less side effects.…”

Section: Bactericidal Coatingsmentioning

confidence: 99%

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Antimicrobial strategies for urinary catheters

Zhu

Wang

et al. 2018

J Biomedical Materials Res

111

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Over 75% of hospital‐acquired or nosocomial urinary tract infections are initiated by urinary catheters, which are used during the treatment of 16% of hospitalized patients. Taking the United States as an example, the costs of catheter‐associated urinary tract infections (CAUTI) are in excess of $451 million dollars/year. The biofilm formation by pathogenic microbes that protects pathogens from host immune defense and antimicrobial agents is the leading cause for CAUTI. Thus, tremendous efforts have been devoted to antimicrobial coating for urinary catheters in the past few decades, and it has been demonstrated to be one of the most direct and efficient strategies to reduce infections. In this article, we briefly summarize the current methods for preparation of antimicrobial coatings based on different stages in the biofilm formation, highlight recent progress in the urinary catheter coating material design and selection, discuss approaches to improving their long‐term antimicrobial efficacy, biocompatibility, multidrug resistance and recurrent infections, and finally outline future requirements and prospects in antimicrobial coating material design. The scope of the works surveyed is confined to antimicrobial urinary catheters. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 445–467, 2019.

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Obliteration of bacterial growth and biofilm through ROS generation by facilely synthesized green silver nanoparticles

Cited by 129 publications

References 31 publications

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Biosynthesis of silver nanoparticle from pomelo (Citrus Maxima) and their antibacterial activity against acidovorax oryzae RS-2

Antimicrobial strategies for urinary catheters

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