Observation of biological samples using a scanning microwave microscope

Park, Jewook; Hyun, Seung Dam; Kim, A.; Kim, T.; Char, K.

doi:10.1016/j.ultramic.2004.09.007

Cited by 32 publications

(14 citation statements)

References 30 publications

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“…NSMMs can be used to study multiphase materials, such as composite and functional materials [3][4], biological materials [5] and minerals [6]. In this paper, this is demonstrated by measurements on particulate rock set in an epoxy and polished.…”

Section: Introductionmentioning

confidence: 99%

Traceable measurement and imaging of the complex permittivity of a multiphase mineral specimen at micron scales using a microwave microscope

Gregory

Blackburn

Hodgetts³

et al. 2017

Ultramicroscopy

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Section: Introductionmentioning

confidence: 99%

Traceable measurement and imaging of the complex permittivity of a multiphase mineral specimen at micron scales using a microwave microscope

Gregory

Blackburn

Hodgetts³

et al. 2017

Ultramicroscopy

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“…Hybrid scanning techniques were explored with the NSMM, and combinations with the scanning tunneling microscope (STM)/ NSMM [6,7] and atomic force microscope (AFM)/NSMM [8,9] were also developed to exploit the tomographic capability of both non-biological [10] and biological materials [11][12][13]. Specialized tips like the GaN nanowire probe [14] were also developed for the NSMM, with the aim of improving the spatial resolution of imaging.…”

Section: Introductionmentioning

confidence: 99%

Imaging of soft material with carbon nanotube tip using near-field scanning microwave microscopy

Wu¹,

Sun²,

Feng³

et al. 2015

Ultramicroscopy

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“…Their work indicated that AFM can be an effective tool for gene/molecule delivery. Furthermore, nowadays, with the development of AFM-based and microwave microscope technology, the THP1 cells (Oh et al 2011) Yucca filamentosa's epidermal cells (Park et al 2005) C2C12 cells (Farina et al 2011(Farina et al , 2012, cancerous breast cells (Tabib-Azar and Wang 2004) have been imaged by scanning microwave microscopy.…”

Section: Introductionmentioning

confidence: 99%

Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale

et al. 2015

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combinations of proteins, carbohydrates, lipids and nucleic acids. These compositions carry out complicated biochemical and physiological process inside the cell. To study these complicated process, acquiring the morphological and fundamental function of these intracellular structures become an indispensable process.With the development of biotechnology, more and more approaches have been developed to investigate the intracellular structures. Immunofluorescence staining was the most widely used method to detect the changes of microfilaments in studying the behavior of cytoskeleton (Tumminia et al. 1998;Xu et al. 2011). Some cellular organelles such as chloroplast have been isolated from plant cells and their morphological structures and functions were investigated in the process of photosynthesis. In order to observe the intracellular structures, special microscopes have been designed. Tokita and cooperators have developed a new kind of microscope that was able to observe the internal structure of living cells (Matsuura-Tokita et al. 2006). Researchers from Caltech tried to observe the arrangement of individual proteins inside cells in a life-like state with the electron crymicroscope (Briegel et al. 2009). Transmission electron microscopy (TEM) and scanning electron microscope (SEM) also play important roles in cellular research.Advances in our understanding of molecular and cellular biology were and still are dictated by the development of new techniques, allowing the structural and functional study of living materials. The nano-scale surface analysis of microbial cells represents a significant challenge of current microbiology and is critical for developing new biotechnological and biomedical applications. The exploration of microbes using atomic force microscopy (AFM) is an exciting research field that has expanded rapidly in the past few years. AbstractIn this paper, we report a non-invasive and nondestructive probing method for analyzing the MG63 osteoblast-like cells. High frequency microwave atomic force microscope (M-AFM) can be used to measure the surface topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation AFM mode, the M-AFM probe tip can scan above the cell surface, maintaining a constant stand-off distance and the created lateral forces were small enough as not to sweep away or deform the fragile biomolecules. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized. The unique potentials of that M-AFM imaging biological substrates with no damaging manner and nanometer scale resolution, while the original structure and function of the biomolecules during the investigation are preserved, make this technique very attractive to biologists.

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Observation of biological samples using a scanning microwave microscope

Cited by 32 publications

References 30 publications

Traceable measurement and imaging of the complex permittivity of a multiphase mineral specimen at micron scales using a microwave microscope

Traceable measurement and imaging of the complex permittivity of a multiphase mineral specimen at micron scales using a microwave microscope

Imaging of soft material with carbon nanotube tip using near-field scanning microwave microscopy

Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale

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