Observation of Peptide-Ion Generation by Laser-Induced Surface Heating from Tungsten Silicide Surfaces

Kim, Shin Hye; Park, Sun Hwa; Song, Jae Yong; Han, Sang Yun

doi:10.5478/msl.2012.3.1.018

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Such rapid surface heating is known to promote the intact desorption of thermally labile molecules from the early field desorption mass spectrometry [12]. Very recently, the thermal aspect involved in the LDI process has been further supported by pronounced LDI of peptides from the flat surfaces of a-Si and WSi x [7,8]. The thermal properties of a-Si and WSi x , such as a very low thermal conductivity (K) and a very high heat capacity (C p ), respectively, make the surfaces to be amenable to laser-induced surface heating, which may replace the needs of the presence of surface nanostructures for LDI of thermally labile molecules from the surfaces.…”

Section: Ldi Of Peptides On Soi Wafers Via Laser-induced Surface Heatingmentioning

confidence: 99%

“…For example, it was recently found that the flat surfaces of amorphous Si (a-Si) [7] and tungsten silicide (WSi x ) [8] yielded pronounced LDI of peptide molecules without assistance of surface nanostructures. As LDI on flat surfaces avoids the utilization of nanostructures that necessitates well-controlled surface fabrication, the use of flat surfaces makes it simpler, easier, and more reproducible to prepare uniform target surfaces.…”

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confidence: 99%

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Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry

Kim

Moon

et al. 2012

J. Am. Soc. Mass Spectrom.

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Abstract. We report here that a commercial silicon-on-insulator (SOI) wafer offers an opportunity for laser desorption/ionization (LDI) of peptide molecules, which occurs directly from its flat surface without requiring special surface preparation. The LDI-on-SOI exhibits intact ionization of peptides with a good detection limit of lower than 20 fmol, of which the mass range is demonstrated up to insulin with citric acid additives. The LDI process most likely arises from laser-induced surface heating promoted by two-dimensional thermal confinement in the thin Si surface layer of the SOI wafer. As a consequence of the thermal process, the LDI-on-SOI method is also capable of creating post-source decay (PSD) of the resulting peptide LDI ions, which is suitable for peptide sequencing using conventional TOF/TOF mass spectrometry.

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Section: Ldi Of Peptides On Soi Wafers Via Laser-induced Surface Heatingmentioning

confidence: 99%

mentioning

confidence: 99%

Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry

Kim

Moon

et al. 2012

J. Am. Soc. Mass Spectrom.

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“…With the goal of optimizing MALDI-MS analysis, extensive efforts have been made to investigate matrix effect, 17,18 matrix additives, 19 sample-matrix preparation methods, [20][21][22] and its mechanism. [23][24][25][26] MALDI-TOF (time-of-flight) MS has become a standard analysis tool for the structural analysis of synthetic polymers. MALDI-TOF MS analysis of synthetic polymers readily provides polymer characteristic information such as the average molar mass (M W ), molar mass distribution (MMD), repeat unit structure, copolymer sequence, and the endgroup.…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF Analysis of Polyhexamethylene Guanidine (PHMG) Oligomers Used as a Commercial Antibacterial Humidifier Disinfectant

Hwang

Nam

Yang

et al. 2013

Bulletin of the Korean Chemical Society

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Polyhexamethylene guanidine (PHMG) polymers used as an active ingredient in an antibacterial humidifier disinfectant were reported to cause harm to the human health when inhaled, although physical contact with this material was known to present low toxicity to humans. It is therefore necessary to develop an optimal analysis method which enables detection and analysis of PHMG polymers. MALDI-TOF investigations of PHMG are performed with a variety of matrices, and it is found that CHCA and 2,5-DHB are excellent matrices which well reflects the polymer population even at high mass. For the provided PHMG sample, the number-average (M n ) and weight-average (M w ) molecular masses were determined to be 744.8 and 810.7, respectively, when the CHCA was used as a matrix. The rank of the matrices in terms of averaged molecular weight was CHCÃ 2,5-DHB > 5-NSA > DHAP, THAP > ATT > IAA ~ super-DHB ~ HABA. In addition, PSD of the PHMG oligomer ions exhibited a few unique fragmenation characteristics. The formation of a-and c-type fragments was the major fragmentation pathway, and the 25-Da loss peaks generally accompanied a-and c-type fragments.

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Rapid Surface Heating Promotes Laser Desorption Ionization of Thermally Labile Molecules from Surfaces

Han¹

2016

Mass Spectrometry Letters

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: In recent years, matrix-free laser desorption ionization (LDI) for mass spectrometry of thermally labile molecules has been an important research subject in the pursuit of new ionization methods to serve as alternatives to the conventional matrixassisted laser desorption ionization (MALDI) method. While many recent studies have reported successful LDI of thermally labile molecules from various surfaces, mostly from surfaces with nanostructures, understanding of what drives the LDI process still requires further study. This article briefly reviews the thermal aspects involved in the LDI mechanism, which can be characterized as rapid surface heating. The thermal mechanism was supported by observed LDI and postsource decay (PSD) of peptide ions produced from flat surfaces with special thermal properties including amorphous Si (a-Si) and tungsten silicide (WSi x ). In addition, the concept of rapid surface heating further suggests a practical strategy for the preparation of LDI sample plates, which allows us to choose various surface materials including crystalline Si (c-Si) and Au tailorable to specific applications.

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Observation of Peptide-Ion Generation by Laser-Induced Surface Heating from Tungsten Silicide Surfaces

Cited by 3 publications

References 13 publications

Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry

Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry

MALDI-TOF Analysis of Polyhexamethylene Guanidine (PHMG) Oligomers Used as a Commercial Antibacterial Humidifier Disinfectant

Rapid Surface Heating Promotes Laser Desorption Ionization of Thermally Labile Molecules from Surfaces

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