Observation of Surface Morphology of Plasma Polymerized Film by Atomic Force Microscopy(AFM).

Sato, Daisuke; Suwa, Toshihiro; Kakimoto, Masa–aki; Imai, Yoshio

doi:10.2494/photopolymer.10.149

Cited by 4 publications

(4 citation statements)

References 3 publications

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“…The surface roughness-related parameter values were similar to those of silicon 22 and mica. 23,24 The overall results mean that the Au surface was completely covered with the PPF. The size exclusion of this layer also serves a role of an antifouling coating against clinically significant oxidizing interferants such as ascorbic-and uric acids previously reported by our group.…”

Section: Resultsmentioning

confidence: 90%

Nanothin Ferrocene Film Plasma Polymerized over Physisorbed Glucose Oxidase: High-Throughput Fabrication of Bioelectronic Devices without Chemical Modifications

2005

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We describe a method for creating a mediator-containing interface between an enzyme and an electrode, achieving simpler and more reliable immobilization of the enzyme with the enhanced detection sensitivity. A nanothin polymer film containing a redox mediator, made of dimethylaminomethylferrocene, was plasma-deposited directly onto a glucose oxidase-physisorbed electrode, with which a relevant bioelectrochemical signal was observed without prior or further chemical modification of the enzyme molecules. The results of the surface characterizations before and after the enzyme immobilization showed that this method gave control over the spatial orientation of single enzyme molecules in favor of efficient and reproducible signal generation. Considering that the film deposition was performed using microfabrication-compatible organic plasma, our new method has a great potential of enabling high-throughput production of bioelectronic devices without chemical modification steps.

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Section: Resultsmentioning

confidence: 90%

Nanothin Ferrocene Film Plasma Polymerized over Physisorbed Glucose Oxidase: High-Throughput Fabrication of Bioelectronic Devices without Chemical Modifications

2005

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“…The HMDS PPF has a variety of three-dimensional cross-linked network conformations with several silicon atoms surrounded by oxygen atoms and methyl groups, creating highly hydrophobic surfaces . The surface roughness-related parameter values ( R rms = 0.21−0.35 nm, Table ) were similar to those of silicon and mica, , indicating that the PPF has a very flatter surface than other polymer surfaces. This surface roughness was not changed with subsequent plasma treatment (non-polymerized monomer gas of nitrogen and oxygen) for surface modification (Table ).…”

Section: Resultsmentioning

confidence: 94%

Adsorption of Glucose Oxidase onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy, Quartz Crystal Microbalance, and Electrochemical Measurement

et al. 2006

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Adsorption of glucose oxidase (GOD) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM), quartz crystal microbalance (QCM), and electrochemical measurements. The PPF surface is very flat (less than 1-nm roughness), and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focused on three types of surfaces: (1) the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic and neutral surface), (2) an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface), and (3) an HMDS PPF surface treated with oxygen plasma (hydrophilic and negative-charged surface). The AFM image showed that the GOD molecules were densely adsorbed onto surface 2 and that individual GOD molecules could be observed. The longer axis of GOD ellipsoid molecules were aligned parallel to the surface, called the "lying position", because of electrostatic association. On surface 1, clusters of GOD molecules did not completely cover the original PPF surface (surface coverage was ca. 60%). The 10-nm-size step height between the GOD clusters and the PPF surface suggests that the longer axes of individual GOD molecules were aligned perpendicular to the surface, called the "standing position". On surface 3, only a few of the GOD molecules were adsorbed because of electrostatic repulsion. These results indicate that the plasma polymerization process can facilitate enhancement or reduction of protein adsorption. The AFM images show a corresponding tendency with the QCM profiles. The QCM data indicate that the adsorption behavior obeys the Langmuir isotherm equation. The amperometric biosensor characteristics of the GOD-adsorbed PPF on a platinum electrode showed an increment in the current because of enzymatic reaction with glucose addition, indicating that enzyme activity was mostly retained in spite of irreversible adsorption.

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“…The PSD intensity of the PPF deposited by 250 W is larger than that deposited by 100 W in the wavelength range between 8 and 60 nm, suggesting that the grain size of the 250-W PPF is slightly larger than that of the 100-W PPF. This tendency was similarly observed in cases of PPFs made from different kinds of monomers. ,, During the course of plasma polymerization, as a matter of fact, the balance among agglomerization, polymerization, and ablation is important as a resultant effect of various plasma generation conditions. As such, this is strongly related to the pore size of the resultant polymer film.…”

Section: Resultsmentioning

confidence: 55%

“…This tendency was similarly observed in cases of PPFs made from different kinds of monomers. 4,26,27 During the course of plasma polymerization, as a matter of fact, the balance among agglomerization, polymerization, and ablation is important as a resultant effect of various plasma generation conditions. As such, this is strongly related to the pore size of the resultant polymer film.…”

Section: Characterization Of Plasma-polymerized Coatings Onmentioning

confidence: 99%

Characterization of Diffusion-Controlled Mass Transport through Nanoporous and Nanothin Films Plasma Polymerized on a Sputtered Platinum Electrode

2005

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We demonstrate the diffusion mode of various redox chemical species through a plasma-polymerized nanothin coating with nanometer-sized pores on a sputtered platinum (Pt) electrode. In this work, hexamethyldisiloxane plasma-polymerized films (PPFs) were added onto the sputtered platinum film, both of which were sequentially deposited in the same vacuum chamber. Results of atomic force microscopy, X-ray photoelectron spectroscopy, and electrochemical studies showed that the PPF provided the platinum electrode with a coating with a complete surface coverage. Sub-nanometer-sized pores (less than 1 nm) responsible for a highly crosslinked polymer network in the PPF coatings offered diffusivity-controlled permeation of redox molecules (i.e., size-exclusivity) rather than solubility-controlled permeation (i.e., chemoselectivity). Consequently, variation of the plasma power could give control over the size of the nanometer-sized cavities.

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Observation of Surface Morphology of Plasma Polymerized Film by Atomic Force Microscopy(AFM).

Cited by 4 publications

References 3 publications

Nanothin Ferrocene Film Plasma Polymerized over Physisorbed Glucose Oxidase: High-Throughput Fabrication of Bioelectronic Devices without Chemical Modifications

Nanothin Ferrocene Film Plasma Polymerized over Physisorbed Glucose Oxidase: High-Throughput Fabrication of Bioelectronic Devices without Chemical Modifications

Adsorption of Glucose Oxidase onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy, Quartz Crystal Microbalance, and Electrochemical Measurement

Characterization of Diffusion-Controlled Mass Transport through Nanoporous and Nanothin Films Plasma Polymerized on a Sputtered Platinum Electrode

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