Observation of the Blood-Brain Barrier Opening by Ultrasound with Microbubbles on Mice Using Intravital Imaging with Two-photon Microscopy

Xia, Lu; Hu, Longyun; Hu, Mengni; Chen, Xin; Chen, Siping; Shen, Yuanyuan

doi:10.1109/ius52206.2021.9593513

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Cited by 3 publications

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“…The common methods for this task include two-photon microscopy and magnetic resonance imaging (MRI) 32,33 . Two-photon microscopy offers in-depth, high-resolution images, and is known for its efficacy in visualizing complex biological processes in vivo.…”

Section: Introductionmentioning

confidence: 99%

Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

Gattegno,

Arbel,

Riess

et al. 2024

Journal of Controlled Release

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Section: Introductionmentioning

confidence: 99%

Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

Gattegno,

Arbel,

Riess

et al. 2024

Journal of Controlled Release

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Higher harmonics dynamic focalization in single-element ring transducers using biaxial driving

Delgado

Curiel

et al. 2023

Ultrasonics

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Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

Gattegno,

Arbel,

Riess

et al. 2023

Preprint

View full text Add to dashboard Cite

Overcoming the blood-brain barrier (BBB) is essential to enhance brain therapy. Here, we utilized nanoscale nanobubbles with focused ultrasound for targeted and improved BBB opening in mice. A microscopy method assessed BBB opening at a single blood vessel resolution employing a dual-dye labeling technique using green fluorescent molecules to label blood vessels and Evans blue brain-permeable dye for quantifying BBB extravasation. A UNET-based deep learning architecture enabled blood vessels segmentation, delivering comparable accuracy to manual segmentation with a significant time reduction. Segmentation outcomes were applied to the Evans blue channel to quantify extravasation of each blood vessel. Results were compared to microbubble-mediated BBB opening, where reduced extravasation was observed in capillaries with 2-6μm diameter. In comparison, nanobubbles yield an improved opening in these capillaries, and equivalent efficacy to that of microbubbles in larger vessels. These results indicate the potential of nanobubbles to serve as enhanced agents for BBB opening, amplifying bioeffects in capillaries while preserving comparable opening in larger vessels.

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Observation of the Blood-Brain Barrier Opening by Ultrasound with Microbubbles on Mice Using Intravital Imaging with Two-photon Microscopy

Cited by 3 publications

References 8 publications

Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

Higher harmonics dynamic focalization in single-element ring transducers using biaxial driving

Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation

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