Observation of Viruses, Bacteria, and Fungi in Clinical Skin Samples under Transmission Electron Microscopy

Ran, Yuping; Hu, Wengying; Zhuang, Kaiwen; Lu, Mao; JinghongHuang,; Xu, Fengni; Xu, Xiu-Lian; Hua, Xia; Lama, Jebina; Ran, Xin; YalinDai,; Song, Lei

doi:10.5772/60957

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Preparation and observation of TEM microscopy samples is as described previously, but with modifications [37,38]. Slices of selected MRSA bacterial samples were fixed by immersion in 2.5% glutaraldehyde (GA) in a 0.1 M sodium cacodylate buffer with pH 7.4 at a temperature of 4 • C for 1 h, then washed in cacodylate buffer.…”

Section: Molecular Assay By Sem and Tem Microscopymentioning

confidence: 99%

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

2021

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Methicillin-resistant Staphylococcus aureus (MRSA) infection is detrimental to hospitalized patients. With diminishing choices of antibiotics and the worry about resistance to colistin in synergistic combined therapy, there are suggestions for the use of herbal derivatives. This investigation evaluated the synergistic effects of Nigella sativa (NS) in combination with beta-lactam (β-lactam) antibiotics on extreme drug-resistant (XDR) MRSA isolates. NS concentrations of 10, 7.5, 5.0, 2.5, 1.0, and 0.1 µg/mL, alone and in combination with β-lactam antibiotics, were used to determine the antimicrobial susceptibility of MRSA isolates by the well diffusion method. Time–kill assays were performed using a spectrophotometer, with time–kill curves plotted and synergism ascertained by the fractional inhibitory concentration (FIC). Scanning and transmission electron microscopy were used to gain insight into the mechanism of action of treated groups. Isolates were inhibited by the NS concentrations, with differences in the zones of inhibition being statistically insignificant at p < 0.05. There were statistically significant differences in the time–kill assay for the MRSA isolates. In addition, NS combined with augmentin showed better killing than oxacillin and cefuroxime. The mechanism of action shown by the SEM and TEM results revealed cell wall disruption, which probably created interference that led to bacterial lysis.

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Section: Molecular Assay By Sem and Tem Microscopymentioning

confidence: 99%

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

2021

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“…is naturally found on the skin surfaces of many animals, including humans. It can cause hypopigmentation or hyperpigmentation on the trunk and other locations in humans 5 . The knowledge about Malassezia spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of Fungi: Malasseziarestricta from Felidae

Hadi

Khalif

2023

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A total of 9 samples of wild cat Felischausfurax (de Winton, 1898) and 13 (11 positives) samples of domestic cat Feliscattus (Linnaeus, 1758) belong to Family Felidae. All cats were trapped and used hair and skin scrapings by forceps and surgical blades. The areas of the collection were: Mosul province (north of Iraq); Baghdad, Al-Rashidiya, Tharthar, Nahrawan, AL-Mahmoudiya (middle of Iraq) and AL-Haretha (south of Iraq). The current study revealed that the sensitive and specific PCR assay allowing rapid and reliable identification of Malasseziarestricta by the fragment size amplified was 500bp in the ITS1 gene in one sample of wild cats. The current study recorded a new strain of Malasseziarestrictathat called AF2013 strain "small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1", complete sequence; and 5.8S ribosomal RNA gene, partial sequence. Which was inserted in GenBank: MW376484.1 from wild cat Felischausfurax for the first time in Iraq. Sequencing revealed close matching of the phylogenetic tree to an isolate from Korea (CP030254). The compression was performed using NCBI – the based nucleotides website. Keywords: Dermatitis, Cutaneous microflora, fungi, Genotype, Malasseziarestricta.

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Observation of Viruses, Bacteria, and Fungi in Clinical Skin Samples under Transmission Electron Microscopy

Cited by 2 publications

References 14 publications

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Molecular analysis of Fungi: Malasseziarestricta from Felidae

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