This study was Conducted in al-muthanna Province/Warka district (It is 25 km from the county seat), On a farm belonging to the Ministry of Agriculture/Agricultural extension department during the season(2019-2020), In order to study the effect of Herbicides and different levels of nitrogen fertilizer on grain yield of barley crop. used four levels of nitrogen: 0, 100, 180 and 280. The second factor of the experiment was the Herbicides treatments, first treatment is used without Herbicide, The second treatment was 2.4.D, and the third treatment use a Topik Herbicide. The experiment was applied by using the R.C.B.D randomized complete block design according to the (splitting) plate arrangement and with three replications. Nitrogen levels were placed in the main panels and Herbicide in the secondary panels. The results are as follows: Herbicide significantly affected all studied traits, As it gave its highest average in the characteristic of ear length, it was 5.64 cm, The number of ears, the number of grains per ear, the average value of 1000 grain weight, and the grain yield, which gave averages of 292.21, 47.03, 34.98 and 2.23, respectively. As for fertilization treatments, they affected the characteristics of the experiment, as production increased by increasing nitrogen fertilization, as the fertilization treatment (N:280) recorded the highest averages in all the studied characteristics.