Observations concerning spleen homotransplantation in normal and irradiated animals

Fisher, Beverly; Fisher, E R

doi:10.1097/00006534-196207000-00098

Cited by 2 publications

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“…That a graft-versus-host reaction could occur was strongly suggested by the pathologic studies in dogs of Dammin and his associates, 13 although their findings were at variance with those reported using similar experimental protocols by Fisher et al 12 . In any case, it has been demonstrated that chronic survival of splenic homografts is possible for as long as seven months in dogs without the wasting characteristics of "runt" or "secondary" disease.…”

Section: Discussionmentioning

confidence: 79%

“…It was noted in the animals, as well as in the three human homografts recovered at autopsy, that intraparenchymal splenic hemorrhage was a prominent feature of rejection, an observation previously made by Fisher and his associates. 12 It seems possible that the sequestration of red cells in the homograft, and the hemolysis which has been observed were features more of repudiation of the splenic homograft than evidence of its inherent ability to destroy host red cells by an immunologic mechanism.…”

Section: Discussionmentioning

confidence: 99%

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Splenic Homotransplantation*

Marchioro¹,

Rowlands²,

Rifkind³

et al. 1965

Annals of the New York Academy of Sciences

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During the past 12 months, five clinical whole-organ splenic homotransplantations have been carried out with the objective of providing active immunologic tissue for the recipient patients. In one case with hypogammaglobulinemia, it was hoped that the transplanted tissue would alleviate a state of immunologic deficiency. In the other four, all of whom had terminal malignancies, the purpose was to superimpose a state of altered immunologic reactivity upon the host in the hope of thereby suppressing the inexorable growth of the neoplasms.As will be described, these procedures can now be judged in each instance to have been without benefit. Nevertheless, full documentation of the cases seems justified not only because of the many implications of transplantation of immunologically competent tissue, but also because of the potentially important observations made during the care of these patients.In addition, a full account will be presented of the supporting canine studies of splenic homotransplantation, inasmuch as many of the principles of clinical therapy and investigation derived from prior observations in the dog. The fact that it is possible to obtain viable splenic homografts in the dog for as long as two-thirds of a year without the production of runt disease or other harmful effects may have application in future research on bone marrow, other lymphoid, or hepatic homografts. Canine Splenic HomotransplantationAll splenic transplants were performed with a method shown in FIGURES 1 and 2. Donors were selected of approximately the same size as the recipient, cooled to 30-32°C., and systemically heparinized (2 mg./kg.). The spleens were revascularized from the pelvic vessels (FIGURE 2) after rotating the organs end-for-end and reversing the anteroposterior relationships (FIGURE 2). By doing this, the eccentrically placed hilum was brought into convenient relation to the blood supply, allowing the long inferior pole to lie comfortably in the left paravertebral gutter without fixation. Physiologically, the preparation differs from normal in that the splenic venous drainage is into systemic rather than portal channels. For controls, autografts were performed.The protocol for splenic homotransplantation is depicted schematically in FIGURE 3.Briefly, experiments were done in which untreated hosts (FIGURE 3A), hosts pretreated with irradiation (FIGURE 3B), and hosts treated with azathioprine (FIGURE 3C) were used. Splenic autograftsSeven dogs received autografts using essentially the same technique and were either sacrificed at nine days (three animals) or followed from eight to ten and one-half months (four animals). Transfusions were not employed in any case. The average hematocrit in the three animals sacrificed after nine days dropped from 46 per cent to 32 per cent. Red cell survival studies were performed on these three dogs, each animal serving as its own control. Before surgery, 10 ml. of autogenous blood was drawn from a foreleg vein, mixed with 4 ml. of ACD solution containing five microcuries of Cr...

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Splenic Homotransplantation*

Marchioro¹,

Rowlands²,

Rifkind³

et al. 1965

Annals of the New York Academy of Sciences

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“…In large animals, most histological observations have been made in dogs (Moore et al . 1960; Fisher et al . 1961; Dammin et al .…”

Section: Discussionmentioning

confidence: 99%

“…Bitter‐Suermann described the morphologic features of SpTx in rats (Bitter‐Suermann & Herbertson 1975), and there have been several studies on outbred dogs (Moore et al . 1960; Fisher et al . 1961; Dammin et al .…”

mentioning

confidence: 99%

Histopathology of spleen allograft rejection in miniature swine

Dor

Gollackner

Kuwaki

et al. 2005

Int J Experimental Path

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Spleen transplantation (SpTx) has established donor-specific tolerance in rodents, but not in large animals or humans. We report the histopathology of rejection in an established model of SpTx in major histocompatibility complex (MHC)-defined miniature swine. Of the 17 SpTx, rejection was observed in two grafts transplanted into untreated, MHC-matched, minor antigen-disparate recipients (group 1, n=4), but not in the two that received a 12-day course of cyclosporin A (CyA). Rejection also occurred in five grafts transplanted into fully MHC-disparate recipients (group 2, n=12), one of which was untreated and four of which received some form of immunosuppressive therapy. One recipient of an MHC class-I-mismatched spleen treated with 12 days of CyA did not show rejection. Following biopsy and/or necropsy, fixed allograft tissue sections were treated with multiple stains, immunohistochemical markers and TUNEL assay. Common features of rejection occurred in grafts from both groups, but with varying time courses. Necrosis developed as early as day 8 in group 2 and day 27 in group 1, ranging from focal fibrinoid necrosis of arteriolar walls and sinusoids to diffuse liquefactive necrosis, usually associated with haemorrhage. Other features of rejection included white pulp expansion by atypical cells and decreased staining of basement membranes and reticular fibres. A doubling of the baseline TUNEL index preceded histologically identifiable rejection. This study establishes histologic guidelines for diagnosing and, perhaps, in future studies, predicting acute rejection of splenic allografts transplanted across known histocompatibility barriers in a large-animal model.

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Observations concerning spleen homotransplantation in normal and irradiated animals

Cited by 2 publications

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Splenic Homotransplantation*

Splenic Homotransplantation*

Histopathology of spleen allograft rejection in miniature swine

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