The present study investigates the ionospheric total electron content (TEC) and F-layer response in the Southern Hemisphere equatorial, low, and middle latitudes due to major sudden stratospheric warming (SSW) event, which took place during January-February 2009 in the Northern Hemisphere. In this study, using 17 ground-based dual frequency GPS stations and two ionosonde stations spanning latitudes from 2.8°N to 53.8°S, longitudes from 36.7°W to 67.8°W over the South American sector, it is observed that the ionosphere was significantly disturbed by the SSW event from the equator to the midlatitudes. During day of year 26 and 27 at 14:00 UT, the TEC was two times larger than that observed during average quiet days. The vertical TEC at all 17 GPS and two ionosonde stations shows significant deviations lasting for several days after the SSW temperature peak. Using one GPS station located at Rio Grande (53.8°S, 67.8°W, midlatitude South America sector), it is reported for the first time that the midlatitude in the Southern Hemisphere was disturbed by the SSW event in the Northern Hemisphere.