Observations on a Newly Recognized Virus (Abney) of the Reovirus Family1

Characterization of the adaptive and innate immune response to intravenous oncolytic reovirus (Dearing type 3) during a phase I clinical trial

“…It is a ubiquitous non-pathogenic agent, isolated from the respiratory and gastrointestinal tracts of humans 2,3 and it has not been associated with a specific clinical syndrome. 4 Reoviruses are selectively able to kill cells with an activated Ras signalling pathway in vitro and in vivo, [5][6][7][8] which can occur through Ras mutation or aberrant expression of upstream mitogenic signals such as overexpressed or mutated receptor tyrosine kinases. Therefore, reovirus represents a potentially useful treatment for a wide variety of solid and haematological tumours including pancreatic, colorectal, thyroid and lung cancers and acute myelogenous leukaemia.…”

Reovirus as a novel oncolytic agent

“…Upon elucidation of the intracellular factors that govern cellular susceptibility, it became clear that reovirus Type 3 Dearing was capable of replicating in cells with an activated Ras signaling pathway, whereas normal, untransformed cells were unable to support reovirus infection (1). Because normal cells are resistant to reovirus, it is not surprising that reovirus infection in humans is usually subclinical (2,3). Altogether, the potential impact of such findings is impressive when one considers that activating mutations in the ras genes alone contribute to more than 30% of all human cancers and that many other mutations in elements of the Ras pathway can also contribute to oncogenesis (4,5).…”

Serological Survey of Laboratory Rodents for Infection With Sendai Virus, Mouse Hepatitis Virus, Reovirus Type 3 and Mouse Adenovirus

“…MHV antigen derived from MHV-2 (Pr) (Fujiwara, 1971) was kindly supplied by Dr. K. Fujiwara, Institute of Medical Science, University of Tokyo. Abney strain of reo 3 (Rosen et al, 1960), obtained from Department of Enteroviruses of this institute, was propagated in primary cultures of cynomolgus monkey kidney (MK) cells. The infected MK cells were freeze-thawed four times and clarified by centrifugation at 3,000 rpm for 15 min.…”