Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Huković, S; Rand, M. J.; Vanov, Svetislav K.

doi:10.1111/j.1476-5381.1965.tb02093.x

Cited by 85 publications

(46 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contraction of the mammalian urinary bladder to parasympathetic nerve stimulation is largely resistant to block by atropine (Langley & Anderson, 1895;Henderson, 1923;Henderson & Roepke, 1934;1935;Ambache, 1955;Ursillo, 1961;Hukovic', Rand & Vanov, 1965;Ambache & Zar, 1970). One explanation of this phenomenon is that the bladder receives a non-cholinergic as well as a cholinergic excitatory innervation (Ambache & Zar, 1970; Dumsday, 1971;Burnstock, Dumsday & Smythe, 1972).…”

Section: Introductionmentioning

confidence: 99%

EFFECTS OF ADENOSINE 5′‐TRIPHOSPHATE (ATP) AND Β‐γ‐METHYLENE ATP ON THE RAT URINARY BLADDER

Brown

Burnstock

Cocks

1979

British J Pharmacology

104

View full text Add to dashboard Cite

High concentrations of adenosine 5′‐triphosphate (ATP, 100 to 1000 μm) were required to cause contraction of the rat urinary bladder, while adenosine and adenosine 5′‐monophosphate (AMP, 1 to 50/im) produced relaxation. One hundred fold lower concentrations of β‐γ‐methylene ATP, which is resistant to degradation to AMP and adenosine, caused dose‐dependent, phasic contractions which mimicked atropine‐resistant responses to nerve stimulation. Adenosine and AMP caused dose‐dependent inhibition of carbachol‐induced contractions; theophylline competitively antagonized this inhibition but not the contractile responses to β‐γ‐methylene ATP, ATP or atropine‐resistant nerve stimulation. These results suggest that the insensitivity of the rat bladder to ATP is due to its rapid degradation to AMP and adenosine and support the hypothesis that the bladder receives a purinergic excitatory innervation.

show abstract

Section: Introductionmentioning

confidence: 99%

EFFECTS OF ADENOSINE 5′‐TRIPHOSPHATE (ATP) AND Β‐γ‐METHYLENE ATP ON THE RAT URINARY BLADDER

Brown

Burnstock

Cocks

1979

British J Pharmacology

104

View full text Add to dashboard Cite

show abstract

“…The reflex control of the urinary bladder depends on several autonomic pathways. Unlike other autonomic organs, such as the pupil or sweat glands, junctional transmission in the bladder of most species is remarkably resistant to atropine whether the stimuli are applied to both pelvic nerves in vivo (Edge, 1955) or by transmural stimulation in vitro (Carpenter, 1963;Hukovic et al, 1965). Furthermore, at low stimulus rates, nerve-induced responses of the bladder are not potentiated by inhibitors of cholinesterase (Ambache & Zar, 1970;Carpenter, 1977;Downie & Dean, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…While the effects of these drugs on nerve-induced bladder contractions have been studied extensively in vivo and in vitro, (Edge, 1855;Ursillo & Clark, 1956;Carpenter, 1963;Hukovic, Rand & Vanov, 1965;Chesher & Thorp, 1965;Downie & Dean, 1977) their effect on micturition by unrestrained animals have not. The reflex control of the urinary bladder depends on several autonomic pathways.…”

Section: Introductionmentioning

confidence: 99%

Atropine and Micturition Responses by Rats With Intact and Partially Innervated Bladder

Carpenter

1981

British J Pharmacology

View full text Add to dashboard Cite

1 Micturition responses by a group of 17 rats were recorded during a water diuresis. During a 2 h period, uniform volumes of urine were passed at regular intervals; the mean of the voiding responses by each animal was consistent from one water loading period to another. Residual urine volumes were physiologically insignificant. 2 Atropine treatment did not compromise seriously micturition by water-loaded rats. Treated animals micturated more frequently; the mean volume was 68% of control. The residual urine volume was equal to that of controls.3 Several weeks after the surgical removal of half the motor innervation of the bladder, there was no significant effect on micturition. Mean voiding volumes were not different from those of controls; residual urine volumes were the same as before denervation. 4 After half the innervation of the bladder had been destroyed, the effect of atropine on micturition was enhanced. Volumes passed were 50% of control; large residual volumes remained when micturition was over. Only in this group could bladder distension be found. 5 It is concluded that functional responses of the rat urinary bladder are not only resistant to atropine but also to the sizeable reduction in the number of neuroeffector units in the bladder itself. The functional reserve of the rat bladder musculature is remarkably high when assessed by its ability to empty adequately.

show abstract

“…It seems probable that hypogastric nerves supplying the guinea-pig urinary bladder are comprised of both prc-and post-ganglionic fibres. It is interesting to note that Hukovic et al, (1965) observed no changes in response of the rat urinary bladder to stimulation of the nerve accompanying the ureter in the presence of a ganglion-blocking agent and they suggested a post-ganglionic innervation in this tissue.…”

Section: Discussionmentioning

confidence: 96%

Ganglionic Blockade by Rhazidine

Bhattacharya¹,

Sk²,

Dk³

1971

Aust J Exp Biol Med

View full text Add to dashboard Cite

Summary. Rhazidine. a monoquaternary alkaloid, exhibited considerable ganglion lilotking property, Responses of the nictitating membrane to pre-gaiiglionic sympathetic .stimulation were abolished without affecting post-ganglionic responses. Responses of the vas deferens to hypogastric nerve stimulation were impaired withotit altering the responses to intramural stimulation in the guinea-pig. Only partial inhibition of response was observed in the guinea-pig urirary bladder to hypogastric nerve stimulation. Spasms induced by nicotine were selectively inhibited in all of the test organs. Effects at adrenergic neuron and post-synaptic receptors were excluded. The results were compared with pentolinium tartarate and indicated specific ganglion blocking efFect of rhazidine.

show abstract

Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Cited by 85 publications

References 18 publications

EFFECTS OF ADENOSINE 5′‐TRIPHOSPHATE (ATP) AND Β‐γ‐METHYLENE ATP ON THE RAT URINARY BLADDER

EFFECTS OF ADENOSINE 5′‐TRIPHOSPHATE (ATP) AND Β‐γ‐METHYLENE ATP ON THE RAT URINARY BLADDER

Atropine and Micturition Responses by Rats With Intact and Partially Innervated Bladder

Ganglionic Blockade by Rhazidine

Contact Info

Product

Resources

About