OBSERVATIONS ON IN VITRO ADRENAL STEROID SYNTHESIS IN THE RAT¹

Androgen Synthesis by the Testes and Adrenal Glands of Rats Poisoned With Cadmium Chloride

“…Earlier studies (Bush, 1953;Hofmann & Davison, 1954;Laplante, Giroud & Stachenko, 1964) have suggested that in the normal rat adrenal gland there is little, if any, ' 17a-hydroxylase ' activity, converting progesterone to 17a-hydroxyprogesterone. Activity has, however, been reported in the regenerated tissue of enucleated adrenals in rats which had developed hyper¬ tension (Brownell, Lee, Beck & Besch, 1963).…”

THE MICRO ESTIMATION OF STEROIDS PRODUCED BY RAT ADRENALS IN VITRO1

“…The same results have been obtained in many other experiments carried out in the same way in which the steroids formed were measured by the UV method only. The failure of Hofmann (1954) to obtain a consistent stimulating effect of ACTH on the steroid production of rat adrenals in vitro is, we believe, due to the lack of calcium in his media (Birmingham et al, 1953). The ratio of the steroid value obtained by the MB method to that obtained by the UV method for every medium analyzed has been invariably less than 1 indicating that the two methods are not measuring entirely the same steroids.…”

The effect of adrenocorticotrophic hormone on the secretion of corticosteroids by the isolated perfused adrenal gland of the dog

“…benzene and one of 5 ml. benzene-ethyl acetate, 5-1; these were then poured through a column of 0-75 g silica gel in benzene (Hofmann & Davison, 1954). The steroids were eluted from the column with 10 ml.…”