Acetylene reduction (AR) rates by cyanobacteria epiphytic on a moss at Marion Island (46°54' S, 37°45' E) increased from-5°C to a maximum at 25 to 27°C. QlO values between 0 and 25°C were between 2.3 and 2.9, depending on photosynthetic photon flux density. AR rates declined sharply at temperatures above the optimum and were lower at 35°C than at 0°C. Photosynthetic photon flux density at low levels markedly influenced AR, and half of the maximum rate occurred at 84 ,umol m2 s-1, saturation occurring at ca. 1,000 ,imol m-2 s-I. Higher photosynthetic photon flux density levels decreased AR rates. AR increased up to the highest sample moisture content investigated (3,405%), and the pH optimum was between 5.9 and 6.2. The addition of P, Co, and Mo, individually or together, depressed AR.