Observations on the binding of testosterone to malignant mammary tumours and other tissues in vitro.

Braunsberg, Hannelore; James, V. H. T.

doi:10.1038/bjc.1967.83

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Following the infusion of 3H-testosterone, none of the six primary tumours appeared to contain markedly greater concentrations of radioactivity than other tissues studied. These findings agree with the results of in vitro incubations in which only one of ten tumours took up labelled testosterone (Braunsberg and James, 1967a). The finding that tissues could not concentrate testosterone from albumin solution in vitro is in agreement with the in vivo result that plasma always contained more free steroid radioactivity than any tissue studied.…”

Section: Discussionsupporting

confidence: 89%

“…In most cases, in which human subjects have been studied, the experimental procedure has been to administer labelled androgen (Deshpande, Bulbrook and Ellis, 1963;Ellis, Parker, Bulbrook and Deshpande, 1965) Table I. ) tive is to describe comparative concentrations of steroids in various tissues, they suffer from the serious disadvantage of requiring complex mathematical analysis, as has been discussed extensively elsewhere (Braunsberg and James, 1967b), and may thus for practical purposes prove incapable of interpretation. Nevertheless, in experimental studies of this type, it has been shown that some malignant breast tumours exhibit higher concentrations of radioactivity than other tissues examined and the conclusion has been drawn that these tumours accumulate testosterone (Deshpande et al, 1963).…”

Section: Discussionmentioning

confidence: 99%

“…It was then dried over anhydrous sodium sulphate and redistilled through a Dufton column. The liquid scintillator was prepared as described in another paper (Braunsberg and James, 1967a).Testosterone-4-14C (101 #tCi/mg. ), uCi/mg.)…”

mentioning

confidence: 99%

“…Received for publication July 25, 1967 STUDIES with human tissue slices in vitro (Braunsberg and James, 1967a) showed that many tissues are capable of binding steroid hormones. Muscle bound relatively little, while adipose tissue showed the largest uptakes of testosterone, oestradiol and progesterone from saline solution.…”

mentioning

confidence: 99%

“…A comparison of individual tissue concentrations in each patient is, therefore, possible. Even when equilibration is incomplete, gross differences in concentrations may be detectable (Braunsberg and James, 1967b).…”

mentioning

confidence: 99%

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A comparison of steroid hormone concentrations in human tissues including breast cancer.

Braunsberg¹,

Irvine²,

James³

1967

Br J Cancer

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STUDIES with human tissue slices in vitro (Braunsberg and James, 1967a) showed that many tissues are capable of binding steroid hormones. Muscle bound relatively little, while adipose tissue showed the largest uptakes of testosterone, oestradiol and progesterone from saline solution. One of ten malignant tumour samples studied took up a relatively large amount of testosterone, but this did not exceed the uptake by adipose tissue. During incubation in serum albumin solution or plasma, little or no steroid was taken up by the tissues studied.The present investigation was undertaken in order to assess whether in vitro equilibration studies can yield meaningful results in relation to tissue hormone concentrations in vivo and to compare the distribution of androgen and oestrogen in human tissues with particular reference to malignant breast tumours. To this end, patients with-or suspected of having-breast cancer were given constant infusions of tracer doses of tritium-labelled testosterone or oestradiol in an attempt to reach radioactive equilibrium in the whole body. Under these conditions, the concentrations of free steroid tritium in the tissues are proportional to those of the endogenous hormone and its metabolites. A comparison of individual tissue concentrations in each patient is, therefore, possible. Even when equilibration is incomplete, gross differences in concentrations may be detectable (Braunsberg and James, 1967b). MATERIALS AND METHODSReagent8.-Petroleum ether (60°-80°) and methylene chloride (both general purpose reagents) were purified as described for hydrocarbons by Bush (1961), except that they were dried over anhydrous sodium sulphate before distillation. Ethanol (Distillers' Company Ltd.) was purified as described for methanol by Bush (1961). Methanol was redistilled and water was glass-distilled. Ether was washed with A volume of saturated aqueous ferrous sulphate acidified with hydrochloric acid, followed by water until neutral. It was then dried over anhydrous sodium sulphate and redistilled through a Dufton column. The liquid scintillator was prepared as described in another paper (Braunsberg and James, 1967a).Testosterone-4-14C (101 #tCi/mg.), uCi/mg.) and oestradiol-6,7-3H (120 #aCi/,ag.) were from the Radiochemical Centre, Amersham, Bucks.Testosterone-1,2-3H (135 ,tCi/,tg.) was kindly provided by the Endocrinology Study Section, National Institutes of Health, Bethesda, Md. The tritium-labelled steroids were stored as dilute solutions (4 to 8 ,uCi/ml.) in dry benzene at -15°C. and were checked for purity by paper chromatography before use and after completion of the last infusion.

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Section: Discussionsupporting

confidence: 89%