Observations on the crystallizable albumin fraction of horse serum

Kekwick, R. A.

doi:10.1042/bj0320552

Cited by 76 publications

(16 citation statements)

References 8 publications

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“…The protein represents whole albumin, and, in analogy with horse serum albumin (25,26), probably contains components of varying carbohydrate content. This is also borne out by the fact that crystalline, carbohydrate-free bovine albumin has already been isolated <10).…”

Section: Discussionmentioning

confidence: 99%

Native and Regenerated Bovine Albumin

Putnam

Erickson

Volkin

et al. 1943

Journal of General Physiology

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Investigation of the effect of denaturation on certain chemical, physicochemical, and biological properties of proteins has proved a valuable approach in the study of protein structure. Further advances have been made by a study of the reversal of the process of denaturation and by comparative measurements of the properties of a protein in the native and regenerated 1 states. Recently, evidence has been given to show that the denaturation of horse serum albumin (2) and of pseudoglobulin (3) by urea and guanldine hydrochloride are essentially irreversible processe s (4-6) although the regenerated proteins simulate certain characteristic properties of the native materials from which they were derived.

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Section: Discussionmentioning

confidence: 99%

Native and Regenerated Bovine Albumin

Putnam

Erickson

Volkin

et al. 1943

Journal of General Physiology

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“…The results are given in Table 1. D2 values could not be calculated for polyglucose, as no value of its diffusion coefficient in water was available. The diffusion coefficient (at 200 C) of serum albumin was taken as 6-1 x 10-7 cm2/sec (Kekwick, 1938), and that of glucose as 5-9 x 10-6 cm2/sec (Friedman & Carpenter, 1939). A single experiment was also done on the diffusion of serum albumin in water and in a gel made by heating a 0-3 % solution of soluble collagen to 370 C; the values of Dx were almost identical, showing that collagen gel of this concentration has little effect.…”

Section: Diffusion Coefficientsmentioning

confidence: 99%

Effects of hyaluronic acid upon diffusion of solutes and flow of solvent

Ogston¹,

Sherman²

1961

The Journal of Physiology

112

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“…New Zealand White male rabbits were injected intravenously with 2.0 ml of injection preparation (1.6 x IOIO viable centres) on Monday, Wednesday and Friday of each week until the antibody agglutination titre for H. inJEuenzae reached 2560. The rabbits were bled 6 days after the last injection by cardiac puncture, and the sera were separated and stored at 2 "C. Due to the bacteriolytic activity of whole antisera, the gamma globulin fractions (antibodies) of the antisera were isolated by sodium sulphate precipitation (Kekwick, 1938) and used in all experiments.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Transformation by Antibodies against Competent Haemophilus influenzae

Bingham

Barnhart

1973

Journal of General Microbiology

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S U M M A R YAntibodies prepared against competent and non-competent Haemophilus injluenzae were compared as to their effect on DNA binding and transformation. The gamma globulin fraction of rabbit antisera prepared against competent bacteria inhibited irreversible binding of DNA and transformation to 2 1 % and 7 %, respectively, of the control values. Antibodies against non-competent bacteria had an insignificant effect on irreversible binding or transformation. The transforming ability of DNA was not altered by exposure to competent organism antibodies. The antibody activity was reduced to one-third of the control value by heating for 10 miii in a boiling water bath. Bacterial agglutinating ability of the antibodies was completely removed by papain treatment, while only 10 %of the antitransforming activity was destroyed. The kinetics and dose-response of antibodies on inhibition of irreversible uptake and transformation are reported. It was concluded that antigenic determinants related to irreversible binding and at least one additional step in the uptake of transforming DNA are integral components of the competent state of H. injluenzae.

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Observations on the crystallizable albumin fraction of horse serum

Cited by 76 publications

References 8 publications

Native and Regenerated Bovine Albumin

Native and Regenerated Bovine Albumin

Effects of hyaluronic acid upon diffusion of solutes and flow of solvent

Inhibition of Transformation by Antibodies against Competent Haemophilus influenzae

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