Observations on the cytochromes of Amoeba proteus, chaos chaos and Tetrahymena geleii

Cytochrome oxidase activity was demonstrated in Amoeba proteus by diaminobenzidine (DAB) cytochemistry. Deposition of the reaction product occurred on the inner mitochondrial membranes and the cristae. The reaction was abolished by cyanide incubations. Positive reactions were produced with both unfixed and fixed cells: although staining potential was destroyed by any prefixatives which included glutaraldehyde. Cells prefixed with 4% formaldehyde, to raise structural preservation, retained staining ability. Amoebae subjected to prolonged anaerobiosis or to treatment with the carcinogen N-methyl-N-nitrosourethane (MNU) displayed a reduction in DAB reactivity. A positive reaction was only produced in incubations of unfixed cells and even in these the intensity of cristal staining was depleted. The possible use of DAB reactions where lesions in mitochondrial functioning have occurred is considered.

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Observations on the cytochromes of Amoeba proteus, chaos chaos and Tetrahymena geleii

Cited by 8 publications

References 6 publications

Die Mitwirkung von Enzymen im Zellstoffwechsel. Konstitution, Kinetik und cytochemische Grundlagen

Die Mitwirkung von Enzymen im Zellstoffwechsel. Konstitution, Kinetik und cytochemische Grundlagen

Der Feinbau der Organoide von Amoeba proteus und seine Beeinflussung durch verschiedene Fixierstoffe

Diaminobenzidine reactions in control and treated amoeba proteus

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