Observations On The Intestinal Carriage Of Pseudomonas Aeruginosa

Stoodle, B. J.; Thom, Bridgette

doi:10.1099/00222615-3-3-367

Cited by 45 publications

(22 citation statements)

References 6 publications

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“…Beyond 1 year, among the 13 infants who were followed up, only 1 eliminated one of the two strains they had previously harbored. These results agree with the frequency noted in adults which varies from 4-6% [1,20,25] to 11 % [19] in normal out-patients and from 18 to 24% [20,21,25] in hospitalized patients.…”

Section: Discussionsupporting

confidence: 82%

Observations on the Intestinal Colonization by <i>Pseudomonas aeruginosa</i> in Newborn Infants

Borderon¹,

Thieffry

Jamet

et al. 1990

Neonatology

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We studied the intestinal flora of 23 newborns, whose meconium had yielded a pure culture of Pseudomonas aeruginosa on blood agar medium. Twelve infants had a single serotype of P. aeruginosa in their meconium, 10 had a second serotype and the last infant was carrying three distinct ones. The maximum levels of P. aeruginosa observed during the first week of life were variable among the infants: 1 · 10³ to 1 · 10¹⁰ CFU/g of stools. The levels diminished progressively afterwards, and after 1 year of age only 1 of the 13 infants examined remained a carrier of P. aeruginosa. In 11 infants a second or a third serotype occurred during the course of the study. The serotypes that appeared secondarily always disappeared before the initial ones. Antibiotics: ampicillin + gentamicin or cefotaxime + netilmicin and colistin which were given to 8 infants had no clear effect on P. aeruginosa levels. Four infants had delayed colonization by Escherichia coli of ≧10 days. All 4 had high levels of P. aeruginosa: 1·10⁷ to 1·10¹⁰ CFU/g stool, and antibiotic therapy, rendering it impossible to assess which was the cause of this delay. This colonization by P. aeruginosa did not lead to any clinical trouble.

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Section: Discussionsupporting

confidence: 82%

Observations on the Intestinal Colonization by <i>Pseudomonas aeruginosa</i> in Newborn Infants

Borderon¹,

Thieffry

Jamet

et al. 1990

Neonatology

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“…Studies in human volunteers contaminated with Pseudomonas aeruginosa in various doses indicated that long-lasting colonization occurred only when ampicillin had been administered (Buck & Cooke, 1969;Stoodley & Thom, 1970). …”

Section: Discussionmentioning

confidence: 99%

Colonization resistance of the digestive tract of mice during systemic antibiotic treatment

Waaij¹,

Berghuis²,

Lekkerkerk³

1972

Epidemiol. Infect.

154

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SUMMARYDuring systemic treatment of mice with ampicillin or streptomycin, oral contaminations with exogenous bacterial species resulted in an abnormal colonization pattern. The contaminants persisted much longer and in much higher concentrations in the caecum of systemically treated mice than in control animals. Spread of the contaminant into the mesenteric lymph nodes and the spleen was found much more often in the antibiotic treated group. This, however, was only seen when the contaminant was ‘resistant’ to the antibiotic injected. The experiments suggest that the ‘CR-inducing species’ of the microflora live in close contact with the mucosa and therefore could be identical with the anaerobic tapered rods described by Savage & Dubos (1968).

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“…When it colonizes the gut, it resides in the small and large intestine. Levels in healthy individuals are typically low (4%) and can increased to >20% in patients with various gastrointestinal diseases (35). Antibiotics that are ineffective against P. aeruginosa have been shown to significantly increase the risk of colonization with this species in intensive care units (36).…”

Section: Discussionmentioning

confidence: 99%

Identification of Pseudolysin (lasB) as an Aciduric Gluten-Degrading Enzyme with High Therapeutic Potential for Celiac Disease

Wei

Tian

Valery

et al. 2015

American Journal of Gastroenterology

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OBJECTIVES Immunogenic gluten proteins implicated in celiac disease (CD) largely resist degradation by human digestive enzymes. Here we pursued the isolation of gluten-degrading organisms from human feces, aiming at bacteria that would digest gluten under acidic conditions, as prevails in the stomach. METHODS Bacteria with gluten-degrading activities were isolated using selective gluten agar plates at pH 4.0 and 7.0. Proteins in concentrated bacterial cell sonicates were separated by diethylaminoethanol chromatography. Enzyme activity was monitored with chromogenic substrates and gliadin zymography. Elimination of major immunogenic gluten epitopes was studied with R5 and G12 enzyme-linked immunosorbent assays. RESULTS Gliadin-degrading enzyme activities were observed for 43 fecal isolates, displaying activities in the ~150–200 and <50 kDa regions. The active strains were identified as Pseudomonas aeruginosa. Gliadin degradation in gel was observed from pH 2.0 to 7.0. Liquid chromatography–electrospray ionization–tandem mass spectrometry analysis identified the enzyme as pseudolysin (lasB), a metalloprotease belonging to the thermolysin (M4) family proteases. Its electrophoretic mobility in SDS–polyacrylamide gel electrophoresis and gliadin zymogram gels was similar to that of a commercial lasB preparation, with tendency of oligomerization. Pseudolysin eliminated epitopes recognized by the R5 antibody, while those detected by the G12 antibody remained intact, despite destruction of the nearby major T-cell epitope QPQLPY. CONCLUSIONS Pseudolysin was identified as an enzyme cleaving gluten effectively at extremely low as well as near-neutral pH values. The potential to degrade gluten during gastric transport opens possibilities for its application as a novel therapeutic agent for the treatment of CD.

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Observations On The Intestinal Carriage Of Pseudomonas Aeruginosa

Cited by 45 publications

References 6 publications

Observations on the Intestinal Colonization by <i>Pseudomonas aeruginosa</i> in Newborn Infants

Observations on the Intestinal Colonization by <i>Pseudomonas aeruginosa</i> in Newborn Infants

Colonization resistance of the digestive tract of mice during systemic antibiotic treatment

Identification of Pseudolysin (lasB) as an Aciduric Gluten-Degrading Enzyme with High Therapeutic Potential for Celiac Disease

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