Observations on the Minor Basic Hemoglobin Component in the Blood of Normal Individuals and Patients with Thalassemia

Kunkel, H. G.; Ceppellini, R; Müller‐Eberhard, Ursula; Wolf, Julius

doi:10.1172/jci103561

Cited by 243 publications

(66 citation statements)

References 12 publications

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“…Kunkel et al (1957) reported that in thalassemia minor hemoglobin electrophoresis demonstrates the predominance of HbA, increased level of HbA2 (3.5-8.0%) and normal or minimally increased level of HbF. Fortova et al (1995) demonstrated range of HbA2 for thalassemia minor between 6.04 ±0.96 %.…”

Section: Different Types Of Anemia Identified By Peripheral Blood Smementioning

confidence: 98%

To Evaluate Anaemia by Erythrocyte Indices, Red Cell Distribution Width and Haemoglobin Electrophoresis With Special Reference to Thalassemia in Paediatric Age Group

Singh¹,

Gautam²

2015

jemds

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Anemia is not a diagnosis; it is a manifestation of an underlying disorder. Thus, even mild, asymptomatic anemia should be investigated so that the primary problem can be diagnosed and treated. Laboratory evaluation begins with a CBC, including WBC and platelet counts, RBC indices and morphology (MCV, MCH, MCHC, RBC distribution width [RDW]), and examination of the peripheral smear. In many instances routine test like Hb, TLC, DLC, GBP fail to decide anemia especially in early cases and also fail to decide the type of anaemia. In such situations the RBC indices and RDW are very useful. These become abnormal even before changes in routine hemogram are appreciable. Thalassemia minor poses problems in diagnosis because GBP reveals no features of hemolysis rather it has microcytic hypochromic picture which has similarity with iron deficiency anemia. It is difficult to differentiate between two by only GBP. Several decision making rules have been proposed for differentiation. METHOD: The present study was carried out in 100 cases to evaluate anaemia in different age groups based on RBC Indices and RDW and to evaluate sensitivity of RBC indices and RDW in diagnosis of anaemia. Cases showing positivity by various rules and RDW in favour of thalassemia minor were subjected to Hb electrophoresis for confirmation of diagnosis. RESULTS: RBC indices are more sensitive for diagnosis of microcytic hypochromic anemia, normocytic normochromic anemia and macrocytic anemia than PBS alone. RDW-CV is superior to all in use to differentiate iron deficiency anemia and thalassemia minor having high sensitivity 87.3% and specificity 90.5%.

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Section: Different Types Of Anemia Identified By Peripheral Blood Smementioning

confidence: 98%

To Evaluate Anaemia by Erythrocyte Indices, Red Cell Distribution Width and Haemoglobin Electrophoresis With Special Reference to Thalassemia in Paediatric Age Group

Singh¹,

Gautam²

2015

jemds

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“…As early as 1955, investigators noted that adult human hemoglobin was heterogeneous (95,96). In 1958, Allen et al (97) reported that with cation-exchange chromatography human hemoglobin could be separated into at least three minor components that had more negative charges than HbA.…”

Section: Glycated Hemoglobin Testingmentioning

confidence: 99%

Tests of Glycemia in Diabetes

et al. 2004

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“…Starch block electrophoresis was carried out as described by Kunkel, Cepellini, Muller-Eberhard and Wolf (13). Column chromatography was performed as described by Allen, Schroeder and Balog (14).…”

Section: Preparative Proceduresmentioning

confidence: 99%