Observations on the Multiplication of Phages Affecting Streptomyces Griseus

Degeneration of a Streptomyces griseus Mutant on Repeated Transfer

“…Study of the morphological types present in the inoculum or fermentation flasks was made by plating a quantity of the vegetative growth on a yeast extract-beef extract agar (Difco) and an agar containing an infusion of soybean meal and glucose. The colonial forms were observed after 5 to 7 days incubation of the agar plates at 25 C. The mutant culture used at the start of the study contained only the asporogenous forms similar to those described by Appleby (1948) and by Koerber, et al, (1950), when grown on the yeast extract-beef extract agar, and sporulating colonies on the soybean infusion agar. The degenerated cultures contained types forming asporogenous colonies on the soybean infusion agar.…”

Electron Microscope Study of the Multiplication of the Actinophage Affecting Streptomyces griseus1

“…The experimental materials were actinophage strains S-1 and Streptomyces griseus strain The methods used in the following experiments resembled in a general way those used by other investigators (Woodruff et al, 1947;Koerber et al, 1950;Perlman et al, 1951).…”

Studies on Streptomyces Phage

“…Parts E and F show the general morphology of phage 514-3. It is evident that the phage of S. griseus resembles in over-all morphology some members of the Escherichia coli series (Williams and Fraser, 1953) and other actinophages (Saudek and Colingsworth, 1947;Woodruff et al, 1947;and Koerber et al, 1950). The average head diameter appears to be 95 to 100 m,u; the tail length, 350 to 360 mu; and tail width, 10 to 15 mI.…”

“…Within recent years, phages capable of infecting various Streptomyces species have been reported (Saudek and Colingsworth, 1947;Reilly et al, 1947;Koerber et al, 1950;Perlman et al, 1951;and Alexander and McCoy, 1956). Hoehn (1949) and subsequently Gilmour and Buthala (1950) demonstrated that streptomyces phage is indigenous to soil, the natural habitat of the host.…”