Observations on the regulation of the synthesis of the tricarboxylic acid cycle enzymes in Bacillus subtilis, Marburg

Hanson, Richard S.; Blicharska, J.; Arnaud, Maryvonne; Szulmajster, Jekisiel

doi:10.1016/0006-291x(64)90416-4

Cited by 37 publications

(31 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…I n bacteria, addition of the amino acid products of the Krebs cycle to the growth medium causes repression of some Krebs cycle enzymes [4,20]. No such repression was found in Neurospora in either sucrose or acetate medium ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

The Regulation of Synthesis of Krebs Cycle Enzymes in Neurospora by Catabolite and End Product Repression

Flavell

Woodward

1970

European Journal of Biochemistry

View full text Add to dashboard Cite

Krebs cycle enzymes were repressed in Neurospora during growth on sucrose as carbon source compared with growth on acetate. In mitochondrial, cytochrome‐deficient strains grown on sucrose, this glucose or catabolite repression was reduced. In mutant strains in which protein synthesis and mitochondrial respiration were inhibited, catabolite repression was enhanced. These data support the hypothesis that ATP or the cellular energy level is involved in catabolite repression. The interrelationships between energy metabolism, the energy distribution in the cell and catabolite repression are discussed. The addition of casamino acids to a culture growing on sucrose or acetate, did not cause repression of any of the Krebs cycle enzymes measured, in spite of the fact that many of the constituent amino acids can be considered as biosynthetic products of the Krebs cycle.

show abstract

Section: Resultsmentioning

confidence: 99%

The Regulation of Synthesis of Krebs Cycle Enzymes in Neurospora by Catabolite and End Product Repression

Flavell

Woodward

1970

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…Since the proteases and a functional TCA cycle appear at about the same time during growth and sporulation of B. subtilis, all of these enzymes may well be under the control of the same effector molecule. Hanson et al (1964) suggested that this molecule might be 'glutamate or a derivative of glutamate'. 'The following model suggests that glutamine plays a key role in regulating these enzymes.…”

Section: Discussionmentioning

confidence: 99%

Continuous Culture Studies on the Biosynthesis of Alkaline Protease, Neutral Protease and -Amylase by Bacillus subtilis NRRL-B3411

Heineken

O’Connor

1972

Journal of General Microbiology

View full text Add to dashboard Cite

The amounts of three catabolite repressible enzymes, alkaline protease, neutral protease and a-amylase, produced by Bacillus subtilis NRRL-~341 I growing in a chemostat, depended on the growth-limiting substrate. Limiting growth with glucose was advantageous for a-amylase synthesis while nitrogen-limited growth was advantageous for synthesis of the two proteases. Under the conditions used, continuous cultures were unsuitable for large-scale production of the three enzymes since spontaneous mutations to less productive strains occurred in the long term. N T R O D U C T I O NContinuous culture studies were initiated by us for two purposes. The first was to develop a better understanding of the regulatory mechanisms involved in the biosynthesis of alkaline protease, neutral protease and a-amylase by Bacillus subtilis NRRL-~341 I. Such information is fundamental to understanding how to alter these mechanisms in order to provide a desired type of micro-organism. The second was to investigate the possibility of synthesizing these enzymes in a large-scale continuous culture. Decided investment advantages (Ellsworth, Telling & East, 1959) of continuous cultures are discussed in the literature as well as some disadvantages (Butterworth, I 967).A number of publications have appeared in recent years on the synthesis of exoenzymes, many of which are reviewed by Schaeffer (1969). Coleman (1967) reported that amylase and proteases of Bacillus subtilis are synthesized in the stationary phase of growth and appear simultaneously and in parallel fashion. Mandelstam (1967) postulates the concept of a catabolite repressing only the enzymes directly or indirectly producing it. We believe we have data which will help to clarify these concepts even further.

show abstract

“…Metabolic alterations that coincide with the onset of sporulation include derepression of tricarboxylic acid (TCA) cycle enzymes, as well as changes in the composition of the electron transport chain (3,7,10,14). Presumably, these changes reflect a need to maintain adequate energy supplies in the absence of a rapidly metabolizable carbon source and constitute a condition necessary for sporulation.…”

mentioning

confidence: 99%

Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis

et al. 1988

View full text Add to dashboard Cite

The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men promoter activity was maximal at the onset of sporulation and declined soon thereafter. These kinetics were similar to the pattern of menaquinone accumulation previously observed. Expression from the men promoter was independent of the presence of the products of the spoOA and spoOH genes and was enhanced by addition of glucose and glutamine to the culture medium. DNA sequence analysis of the promoter region revealed a potential recognition site for the principal vegetative form of RNA polymerase but not for any of the known minor polymerase forms. The (Fig. 1). A men'-lacZ transcriptional gene fusion was created by cloning this fragment into the integrable lacZ fusion vector pDEB1 (31). The structure of this plasmid, pAI103, is shown in Fig. 2. Strains containing chromosomally integrated single copies of pAI103 were generated by transformation of appropriate recipients and selection for chloramphenicol resistance.

show abstract

Observations on the regulation of the synthesis of the tricarboxylic acid cycle enzymes in Bacillus subtilis, Marburg

Cited by 37 publications

References 7 publications

The Regulation of Synthesis of Krebs Cycle Enzymes in Neurospora by Catabolite and End Product Repression

The Regulation of Synthesis of Krebs Cycle Enzymes in Neurospora by Catabolite and End Product Repression

Continuous Culture Studies on the Biosynthesis of Alkaline Protease, Neutral Protease and -Amylase by Bacillus subtilis NRRL-B3411

Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis

Contact Info

Product

Resources

About