Observations on the Substrate Specificity of Amine Oxidases

Abstract

A number of compounds have been tested as substrates of, first, the amine oxidases of rabbit and guinea-pig liver and of goat, pig, horse, and dog plasma and, second, the " diamine oxidase " of pig kidney. Of the three xylylenediamines [di(aminomethyl)benzenes] tested, m-xylylenediamine was found to be a substrate of the liver oxidase. All the plasma oxidases tested acted on both m-and o-xylylenediamine. Both 3-and 4-picolylamine (3-and 4-aminomethylpyridine) were readily oxidized by the liver enzymes; all the plasma oxidases tested oxidized 2-, 3-, and 4-picolylamine. 4-Picolylamine was more rapidly oxidized by horse and dog plasma than any other substrate hitherto examined.Recent studies on the amine oxidases of mammalian plasma (Blaschko and Hawes, 1959; Blaschko, Friedman, Hawes, and Nilsson, 199) have shown that the amine oxidases in general are a class of enzymes with a great diversity of substrate specificity. The inhibitor specificities of the plasma oxidases show that they are more closely related to the classical histaminase of pig kidney, although their substrate specificity is in many ways reminiscent of that of the intracellular amine oxidase.

A reinvestigation of the substrate specificity of pig kidney diamine oxidase

“…There is good agreement with literature values, except for compound (XI), which we found to be a good substrate, butwhich was reported by Blaschko & Chrusciel (1959) to be unoxidized, and compounds (XVI) and (XVII), which were not oxidized as well as is reported (Zeller et at. 1956).…”

“…The enzyme known as pig kidney diamine oxidase or histaminase [diamine-oxygen oxidoreductase (deaminating), EC 1.4.3.6] was first reported by McHenry & Gavin (1932), and the substrate specificity was extensively investigated by Zeller, Fouts, Carbon, Lazanas & Voegtli (1956), Fouts, Blanksma, Carbon & Zeller (1957), Blaschko & Chrusciel (1959) and Zeller (1963). The enzyme has since been obtained in a homogeneous form (Mondovi et al 1967) and crystallized (Yamada, Kumagai, Kawasaki, Matsuii & Ogata, 1967).…”