Observations on the Tube Method of Measuring Growth Rate in Neurospora crassa

Gillie, O. J.

doi:10.1099/00221287-51-2-185

Cited by 10 publications

(5 citation statements)

References 4 publications

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“…of the initial glucose concentration when this is the growth limiting nutrient. Gillie (1968) found that the rate of extension of Neurospora crassa colonies was proportional to the log. of the arginine concentration over the lower range, and it would thus seem that this is a general relationship for any growth limiting nutrient.…”

Section: Discussionmentioning

confidence: 99%

A Kinetic Study of the Growth of Aspergillus nidulans and Other Fungi

Trinci

1969

Journal of General Microbiology

276

122

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The growth kinetics of Aspergillus nidulans, Mucor hiemalis and Penicillium chrysogenum on solid media and in submerged culture were studied. Growth of A. nidulans colonies on solid medium can be divided into the four phases of lag, exponential, deceleration and constant growth rate. The growth kinetics of A. nidulans in submerged culture were similar to those commonly found for unicellular micro-organisms. The effects on colony radial growth rate, of glucose concentration, medium depth, oxygen partial pressure and temperature were studied. The radial growth rate of glucose-limited colonies of A. nidulans increased linearly with the log. of the initial glucose concentration. The effects of glucose concentration on internode length, hyphal density and hyphal diameter were also studied. At glucose concentrations above I % (wlv) there was an inverse relation between the radial growth rate of A. nidulans colonies and their peripheral hyphal density. The relations between colony radial growth rate (K,) and germ tube specific growth rate (ag) on solid media and specific growth rate in submerged culture (a,) were investigated. Direct proportionality between K, and a, of A. niduZans was found by varying specific growth rate by temperature changes. The germ tubes of A. nidulans conidia grew exponentially at a rate which was about 2.3 times as great as the specific growth rate of the organism in submerged culture. The colony radial growth rates of the three moulds could not be used as a measure of their relative specific growth rates in submerged culture.

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Section: Discussionmentioning

confidence: 99%

A Kinetic Study of the Growth of Aspergillus nidulans and Other Fungi

Trinci

1969

Journal of General Microbiology

276

122

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“…The rate of linear extension of a mycelial front of wild-type Neurospora can be as great as 0.6 cm/h (105). Although there are many conditions, such as nonoptimal temperature and genetic constitution, that will markedly diminish this rate (38,57), the rate of advance of a mycelial front on a solid medium usually depends surprisingly little on nutrition. It is the density of branching behind the front that varies dramatically (56,57).…”

Section: Miscellaneous Underexploitedmentioning

confidence: 99%

“…Although there are many conditions, such as nonoptimal temperature and genetic constitution, that will markedly diminish this rate (38,57), the rate of advance of a mycelial front on a solid medium usually depends surprisingly little on nutrition. It is the density of branching behind the front that varies dramatically (56,57). Hence, on a rich, mediocre, or poor medium, Neurospora can generally push forward a mycelial front at a rate of 10 to 14 cm/ day.…”

Section: Miscellaneous Underexploitedmentioning

confidence: 99%

Implications of some genetic control mechanisms in Neurospora.

Metzenberg¹

1979

Microbiological Reviews

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INEODUCTION Apologia For the would-be writer of a review article, it can be sobering to ask whether the article is needed. On posing this question to myself, I realized that a number of very perceptive and, in some cases, extensive reviews have already appeared in print. These are listed below. I saw few ways to cover the same ground differently, and certainly no way to cover it better. It seemed more profitable to use this space as an opportunity (i) to examine a few of the special features of Neurospora crassa and closely related species that are likely to escape attention elsewhere and (ii) for some unabashed speculation about the evolutionary pressures that have shaped the control mechanisms extant in Neurospora and perhaps in other eucaryotes. This article is meant to be read with that in mind. Books, Reviews, and Other Sources of Information The following sources will be useful to those wishing to get abreast of the literature of Neurospora. Fincham and Day (45). General fungal genetics textbook, with emphasis on Neurospora. Chromosome mechanics, mutagenesis, mapping functions, fine structure determination, extrachromosomal inheritance, physiological genetics. Davis (37). A critical review of metabolic organization and compartmentation of function in Neurospora and other fungi. Prevention of futile cycling by summation of anabolism and catabolism. Marzluf (89). A valuable discussion of genetic regulation of metabolic pathways in fungi. Perkins and Barry (103). A remarkable, comprehensive review of cytogenetics, meiosis and mitosis, and chromosomal aberrations; life cycle of N. crassa and related heterothallic and pseudohomothallic species. Contains genetic maps and tabulations of kinds of auxotrophs and morphological, color, and other kinds of mutants. Perkins et al. (105). Natural history of the genus Neurospora. Taxonomy and chromosome homology of a large collection of isolates from nature. Srb et a1. (121). Genetics of development of the sexual apparatus. Nelson et al. (98). Biochemical and morphological changes during conidium formation, especially as revealed by mutational dissection. Schmit and Brody (116). Biochemistry and physiology of conidial germination. Brody (20) and Scott (117). Effect of enzyme levels and metabolic pools on cell wall and membrane composition and on morphology. Mishra (95). Various aspects of morphogenesis. Bachmann and Strickland (12). An inval-361

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“…This was made possible by an apparently sparse growth. Gillie (1968) weighed sections of mycelium of Neurospora crassa after extraction of agar and freeze-drying. Pittendrigh et al (1960) used a densitometer for optically scanning density striations in N .…”

Section: E D E L S T E I N a N D Others Discussionmentioning

confidence: 99%

“…The method chosen after some experimentation was a modification of Gillie's (1968) direct weighing procedure. Our modifications included (a) growing the colonies on cellophane-covered media, hence eliminating the need for agar extraction, (b) removing mycelia from the growth surface with sticky tape, enabling a more accurate dissection of fixed areas, and (c) repeating measurements for different growth times, hence obtaining a time sequence of the colony growth.…”

Section: E D E L S T E I N a N D Others Discussionmentioning

confidence: 99%

A Model for Fungal Colony Growth Applied to Sclerotium rolfsii

et al. 1983

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A model which relates branching kinetics to colony shape in filamentous fungi was used to study the growth of Sclerotium rolfsii. Model variables include the densities of hyphae and tips along the radius of the colony. Experiments designed to measure these variables revealed peaked distributions of hyphae, and tip densities which were concentrated just within the margin of the colony. The phenomena of lateral branching, hyphal autolysis, and anastomosis, which are known to occur in S . rulfsii, are incorporated into the model. Predictions generated by the model agree with experimental observations on this species.

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Observations on the Tube Method of Measuring Growth Rate in Neurospora crassa

Cited by 10 publications

References 4 publications

A Kinetic Study of the Growth of Aspergillus nidulans and Other Fungi

A Kinetic Study of the Growth of Aspergillus nidulans and Other Fungi

Implications of some genetic control mechanisms in Neurospora.

A Model for Fungal Colony Growth Applied to Sclerotium rolfsii

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