Observed energy intake of weaned piglets and its effect on temperature requirements

Robertson, Anne M.; Clark, J. J.; Bruce, J. M.

doi:10.1017/s0003356100040162

Cited by 15 publications

(8 citation statements)

References 2 publications

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“…In vitro pancreatic lipase activity is inhibited by bile salts and its activity is restored by the addition of colipase. 28 Other studies in patients with steatorrhea also found that the degree of fat malabsorption is inversely proportional to the colipase concentration in the pancreatic secretions. 25 Collectively, these previous results showing that colipase has a significant effect on lipase lipolytic activity in vitro and in vivo suggested that colipase is essential for pancreatic lipase activity under physiological conditions.…”

Section: Discussionmentioning

confidence: 94%

Application of Porcine Lipase Secreted by Pichia pastoris to Improve Fat Digestion and Growth Performance of Postweaning Piglets

Liu

Chen

Lin

et al. 2010

J. Agric. Food Chem.

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The aim of the study was to use Pichia pastoris to express a recombinant porcine lipase gene (pLip). The expression-secretion cassette was constructed using the P. pastoris GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) promoter and an 89-residue prepro-alpha-factor secretion signal fused to the AOX1 terminator (the pGAPZalphaA vector). A total of 1,408 bp of pancreatic lipase cDNA was produced, which was located from the position of 4-nt upstream of ATG to 1408-nt inside the intact coding region of the pLip sequence. In an animal trial, three concentrations of recombinant lipase activity (0, 5,000 and 10,000 U/kg) were blended with the basal diet and fed to weaned piglets for six weeks. During the experimental period, the growth performance (bodyweight, feed intake, and feed efficiency) of the test groups was superior to that of the control group (p < 0.05). Furthermore, the group fed the diet blended with 10,000 U/kg of recombinant lipase showed significant (p < 0.05) increases in blood triglyceride (TG) concentration on the seventh day postweaning. These results suggested that the porcine lipase protein yielded by transformed yeast cells may improve fat digestibility and enhance the growth performance in postweaning piglets.

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Section: Discussionmentioning

confidence: 94%

Application of Porcine Lipase Secreted by Pichia pastoris to Improve Fat Digestion and Growth Performance of Postweaning Piglets

Liu

Chen

Lin

et al. 2010

J. Agric. Food Chem.

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“…Hampson, 1983), and only Kelly et al (1991c) have examined effects of a controlled level of food intake on gut structure and function. Some workers (Robertson, Clark and Bruce, 1985;Bark, Crenshaw and Leibbrandt, 1986) have shown that after weaning there is a period of temporary starvation during which piglets fail to eat sufficient food to cover their maintenance energy requirement. McCracken (1984) and McCracken and Kelly (1984) suggested that villous atrophy and reductions in digestive enzyme activity after weaning may be related more to the lack of a continuous supply of substrate than to any antigenicity of the diet or inherently low levels of disaccharidase activity.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of villous height and crypt depth in piglets by providing continuous nutrition after weaning

1996

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(3) and (4) (P < 0-05 and P = 0-073, respectively). In turn, villous height was significantly correlated (r = 0-78 to 0-87, P < 0-05) with the rate of bodyweight gain after weaning in these two groups. For piglets offered ewes' milk plus glutamine, an increase in DM intake was associated only with increases in crypt depth (P < 0-01). These data show that the structure and function of the small intestine can be preserved when a milk diet is given after weaning, and suggest an association between food intake and villous height in determining post-weaning weight gain. were offered ewes' fresh milk every 2 h in a feeding schedule that increased from 1-2 I per piglet on the 1st day after weaning to 2-4 I on days 4 and 5. On the 5th day all piglets were killed and samples of small intestine were taken for histological and biochemical examination. Feeding ewes' milk or ewes' milk plus 20 g L-glutamine per I maintained (P > 0-05) villous height and crypt depth compared with piglets killed at weaning. In contrast, piglets given a dry starter diet had shorter villi (P < 0-001), deeper crypts (P < 0-001), and proportionately 0-21 to 0-28 less protein (P>0-05) in their intestinal mucosa. Piglets given the starter diet proportionately grew from 0-49 to 0-62 more slowly (P < 0-01), ate the same amount of dry matter (DM; P > 0-05), but consumed proportionately 0-30 less energy (P < 0-001) than their counterparts given the milk diets. No treatment differences in the specific activity of lactase and sucrase were observed (P>0-05). Significant correlations existed between voluntary food intake and villous height at the proximal jejunum for piglets given the starter diet and ewes' milk

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“…Additionally, porcine colipase contains five disulfide bridges within its 3D structure, which may function by promoting a tight core structure within the molecule. In vitro pancreatic lipase activity is inhibited by bile salts and its activity is restored by the addition of colipase 28. Other studies in patients with steatorrhea also found that the degree of fat malabsorption is inversely proportional to the colipase concentration in the pancreatic secretions 25.…”

Section: Discussionmentioning

confidence: 93%

Production of recombinant porcine colipase secreted by Pichia pastoris and its application to improve dietary fat digestion and growth of postweaning piglets

Liu

Chen

Chong

et al. 2008

Biotechnology Progress

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Recombinant porcine pancreatic colipase (pCoL) was produced in the methylotrophic yeast Pichia pastoris. A synthetic yeast secretion cassette was constructed with the constitutive promoter of the glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) gene and the yeast a-mating factor signal peptide. The pCoL cDNA corresponding in the coding sequence, excluding a 16-amino acid segment of the native signal sequence, was cloned into the pGAPZaB vector and integrated into the genome of P. pastoris. Yeast transformants were cultured and bioactive pCoL protein was detected in the supernatant at a high-level of 126.8 mg/L after 3 days of culture. The transformed yeast containing the highest recombinant colipase level (pCoL yeast) and native yeast GS 115 not containing pCoL (non-pCoL yeast, as a control group) were separately cultured and the supernatants were adsorbed by dried skim milk. In an animal trial, two concentrations of colipase activity (0 vs. 5,000 U/kg in the diet) were blended with the pig corn-soybean basal diet and fed to weaned piglets for 4 weeks. The pCoL-administrated test group gained significantly more weight than piglets in the control group when measured at Day 15 (11.84 AE 0.70 vs. 10.59 AE 0.39 kg, P \ 0.05), Day 22 (15.84 AE 0.95 vs. 14.32 AE 0.59 kg, P \ 0.01), and Day 28 (20.19 AE 1.47 vs. 18.54 AE 0.92 kg, P \ 0.01) after weaning. The blood triglyceride (TG) concentrations were significantly increased in the experimental test group that received recombinant colipase on the 28th day of postweaning when compared with that of the control group (32.50 vs. 16.37 mg/dL; P \ 0.0001). These experimental data suggest that the use of recombinant porcine colipase as a dietary supplement provides an alternative approach for improving fat digestion and enhancing growth in postweaning piglets.

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Observed energy intake of weaned piglets and its effect on temperature requirements

Cited by 15 publications

References 2 publications

Application of Porcine Lipase Secreted by Pichia pastoris to Improve Fat Digestion and Growth Performance of Postweaning Piglets

Application of Porcine Lipase Secreted by Pichia pastoris to Improve Fat Digestion and Growth Performance of Postweaning Piglets

Maintenance of villous height and crypt depth in piglets by providing continuous nutrition after weaning

Production of recombinant porcine colipase secreted by Pichia pastoris and its application to improve dietary fat digestion and growth of postweaning piglets

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