Obstetric Ultrasonography to Detect Fetal Abnormalities in a Mouse Model for Zika Virus Infection

Abstract

In 2015 Zika virus (ZIKV) emerged for the first time in South America. The following ZIKV epidemic resulted in the appearance of a clinical phenotype with microcephaly and other severe malformations in newborns. So far, mechanisms of ZIKV induced damage to the fetus are not completely understood. Previous data suggest that ZIKV may bypass the placenta to reach the fetus. Thus, animal models for ZIKV infection are important to facilitate studies about ZIKV infection during pregnancy. Here, we used ultrasound based imaging (USI) to characterize ZIKV induced pathogenesis in the pregnant Type I interferon receptor-deficient (IFNAR-/-) mouse model. Based on USI we suggest the placenta to be a primary target organ of ZIKV infection enabling ZIKV spreading to the fetus. Moreover, in addition to direct infection of the fetus, the placental ZIKV infection may cause an indirect damage to the fetus through reduced uteroplacental perfusion leading to intrauterine growth retardation (IUGR) and fetal complications as early as embryonic day (ED) 12.5. Our data confirmed the capability of USI to characterize ZIKV induced modifications in mouse fetuses. Data from further studies using USI to monitor ZIKV infections will contribute to a better understanding of ZIKV infection in pregnant IFNAR-/- mice.

Assessing the role of Ndel1 oligopeptidase activity in congenital Zika syndrome: Potential predictor of congenital syndrome endophenotype and treatment response

“…Further studies, aiming to better understand these mechanisms, are clearly crucial to create new strategies to prevent and treat the outcomes following the infections by the already known pathogens, such as the ZIKV, or by emerging pathogens as the SARS-CoV2 (Hessami et al, 2022). Although several efforts to identify repositioning or investigational drugs for ZIKV infection treatment (Giovannoni et al, 2020;Li et al, 2019;Sariyer et al, 2019;Viveiros Rosa et al, 2020), there is no good biomarkers for the early diagnosis of congenital ZIKV infection syndrome (CZS) that can lead to microcephaly and which diagnosis is still limited to the measurement of the brain size by ultrasonography or tomography, or by the imprecise measurements of the circumference of the baby's skull after birth (Forster et al, 2020;Gullo et al, 2022). controls (Gadelha et al, 2013), in addition to an even lower Ndel1 activity in treatment resistant compared to non-resistant schizophrenia patients (Gadelha et al, 2013).…”