Obtaining and analysis of isopropyl‐N‐phenyl carbamate resistant lines of Nicotiana species

Effects of the herbicide isopropyl‐N‐phenyl carbamate on microtubules and MTOCs in lines of Nicotiana sylvestris resistant and sensitive to its action

“…Previously, we described the influence of IPC on growth, morphology and division of cells in IPC-resistant (mutant) and IPC-sensitive (control) N. sylvestris lines (Yemets et al, 2003. We found in control plants that IPC treatment results in a swollen root tip phenotype and an appearance of multipolar cells, whereas mutant plants were resistant to its action.…”

“…To clarify the mechanism of IPC action on higher plant cells we have studied the sensitivity of MTs (cortical network and mitotic arrays) to IPC treatment in IPC-resistant and sensitive Nicotiana sylvestris lines (Yemets et al, 2003;Stelmakh et al, 2005). Here we also report the effect of IPC on MTOCs distribution in interphase and dividing mutant and control plant cells.…”

Effect of 2,4-D and isoproturon on chromosomal disturbances during mitotic division in root tip cells of Triticum aestivum L.

“…11, 22). Microtubule spoiling herbicides have an immense prospective in screening for mutant plants with changed herbicide sensitivity in treated plants [67,68]. It could play a remarkable role for agricultural significance worldwide [69,70].…”

Early PCD events in male gametophyte development of isopropyl-N-phenyl carbamate resistant Nicotiana sylvestrismutant lines

“…A better understanding of the processes coupling microtubule damage to the onset of apoptosis will help to find out in process of higher plants development. Recently, the mutant Nicotiana lines with high resistance to antimitotic herbicide isopropyl- N -phenyl carbamate (IPC) were produced [2,3]. Detailed analysis of IPC-resistant Nicotiana sylvestris plants revealed some abnormalities in microsporogenesis leading to partial pollen sterility.…”