Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

Şen, Ayşe; Sarsu, Fatma

doi:10.23902/trkjnat.571255

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Different approaches has been used in the past to develop wheat mutants like using EMS or gamma irradiation in order to develop lines with very high tolerance level. The selected mutants were further used for the back cross breeding program in order to develop genotypes with high tolerance level 16 . The generation of desirable donor lines using the approach of mutation has helped the breeder in the development of climate smart crop in the past.…”

Section: Introductionmentioning

confidence: 99%

Insight into the mechanisms of terminal HS-tolerance in wheat mutant with improved nutritional quality through de novo transcriptome sequencing

Kumar

Bakshi

Dubey

et al. 2021

Preprint

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Terminal HS is one of the main bottle-neck in wheat yield and grain-quality. Here, we have developed wheat mutant (M3) for HS-tolerance [parent-MP3054- C-306/CB.SPRING BW/CPAN2072 (Parentage)]. To elucidate the mechanism of thermotolerance in mutant, we performed de novo transcriptomic sequencing of mutant (M3), parent (P3), and mutant exposed to HS (M3H). We sequenced 6.5, 7.5, and 7.0 million reads in P3, M3 and M3H and generated 3,05,537 genes and 5,88,788 transcripts with an N50 of 1,349 bp. We observed 6,120 upregulated and 4,428 downregulated transcripts (M3 vs P3), 11,354 upregulated and 12,408 downregulated genes (M3H vs P3) and 4817 upregulated and 9085 downregulated genes (M3H vs M3). Some of the highly upregulated genes observed were HSP20, SOD, ABC transporters, HSF, etc. and downregulated genes were starch synthase, sucrose synthase, etc. Gene Ontology analysis showed ‘ATP-binding’ to be most enriched category. Carbon metabolism pathway was observed most altered under HS. We identified 41940 SSRs, 1,10,772 SNPs and 2432 InDels. Potential markers were observed lying on HSP, SOD, STK, and starch synthase. Biochemical markers based characterization showed wheat mutant to be better in HS-tolerance and grain-quality, as compared to parent.

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Section: Introductionmentioning

confidence: 99%

Insight into the mechanisms of terminal HS-tolerance in wheat mutant with improved nutritional quality through de novo transcriptome sequencing

Kumar

Bakshi

Dubey

et al. 2021

Preprint

View full text Add to dashboard Cite

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Involvement of Genetic Mutations in Plant Salt Tolerance

Sheraz,

Ramzan,

Shokat

2024

Genetics of Salt Tolerance in Plants

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Differentiation of advanced generation mutant wheat lines: Conventional techniques versus Raman spectroscopy

et al. 2023

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This research aimed to assess the feasibility of utilizing Raman spectroscopy in plant breeding programs. For this purpose, the evaluation of the mutant populations set up the application of 4 mM NaN3 to the somatic embryos obtained from mature wheat (Triticum aestivum L. Adana-99 cv.) embryos. Advanced wheat mutant lines, which were brought up to the seventh generation with salt stress tolerance by following in vitro and in vivo environments constructed by mutated populations, were evaluated using conventional techniques [measurement of antioxidant enzyme activities (SOD, CAT, and POX), total chlorophyll, TBARS, and proline contents; measurement of the concentration of Na+ and K+ ions; and evaluation of gene expression by qPCR (TaHKT2;1, TaHKT1;5, TaSOS1, TaNa+/H+ vacuolar antiporter, TaV-PPase, TaV-ATPase, and TaP5CS)] and Raman spectroscopy. In this research, no significant difference was found in the increase of SOD, CAT, and POX antioxidant enzyme activities between the salt-treated and untreated experimental groups of the commercial cultivar, while there was a statistically significant increase in salt-treated advanced generation mutant lines as compared to control and the salt-treated commercial cultivar. Proline showed a statistically significant increase in all experimental groups compared to the untreated commercial cultivar. The degradation in the amount of chlorophyll was lower in the salt-treated advanced generation mutant lines than in the salt-treated commercial cultivar. According to gene expression studies, there were statistical differences at various levels in terms of Na+ and/or K+ uptake from soil to plant (TaHKT2;1, TaHKT1;5, and TaSOS1), and Na+ compartmentalizes into the cell vacuole (TaNa+/H+ vacuolar antiporter, Ta vacuolar pyrophosphatase, and Ta vacuolar H+-ATPase). The expression activity of TaP5CS, which is responsible for the transcription of proline, is similar to the content of proline in the current study. As a result of Raman spectroscopy, the differences in peaks represent the protein-related bands in mutant lines having a general decreasing trend in intensity when compared to the commercial cultivar. Amide-I (1,630 and 1,668 cm−1), Histidine, Lysine, Arginine, and Leucine bands (823, 849, 1,241, 1,443, and 1,582 cm−1) showed decreasing wavenumbers. Beta-carotene peaks at 1,153 and 1,519 cm−1 showed increasing trends when the normalized Raman intensities of the mutant lines were compared.

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Obtaining Candidate Salt Tolerant Wheat Mutant Lines Derived From Combination of Sodium Azide Mutagenesis and Somatic Embryogenesis

Cited by 3 publications

References 13 publications

Insight into the mechanisms of terminal HS-tolerance in wheat mutant with improved nutritional quality through de novo transcriptome sequencing

Insight into the mechanisms of terminal HS-tolerance in wheat mutant with improved nutritional quality through de novo transcriptome sequencing

Involvement of Genetic Mutations in Plant Salt Tolerance

Differentiation of advanced generation mutant wheat lines: Conventional techniques versus Raman spectroscopy

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