Obtaining farm animal embryos &lt;i&gt;in vitro&lt;/i&gt;

Duszewska, A. M.; Rapala, L.; Trzeciak, P.; Dąbrowski, Sebastian; Piliszek, Anna

doi:10.22358/jafs/66068/2012

Cited by 6 publications

(7 citation statements)

References 92 publications

(135 reference statements)

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“…Embryo transfer (ET) can be used in animal breeding (Duszewska et al, 2012), mainly as a part of a cattle breeding system (Merton et al, 2003). Stádník et al (2013) reported a growing trend of ETs performed worldwide, however, significantly reduced ET cycles have been recorded in the Czech Republic.…”

Section: Discussionmentioning

confidence: 99%

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

Stádník¹,

Makarevich²,

Doležalová³

et al. 2014

J. Anim. Feed Sci.

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Section: Discussionmentioning

confidence: 99%

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

Stádník¹,

Makarevich²,

Doležalová³

et al. 2014

J. Anim. Feed Sci.

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“…The last stage IVP is known as in vitro culture. That is the development of the embryos from zygote to the morula and blastocyst stages (Duszewska et al, 2012). According to different authors (Lonergan & Fair, 2008;Paramio, 2010;Dang-Nguyen et al, 2011), this encompasses nuclear and cytoplasmic modifications and their mutual interactions.…”

Section: Principles Of In Vitro Embryo Productionmentioning

confidence: 99%

“…In vitro embryo production (IVEP) is one of the most crucial techniques amongst embryo biotechnologies used in farm animal breeding since it does not only help in production of high genetic merit animals, but also serves as an excellent source of embryos for emerging biotechnologies like embryo sexing, cloning, nuclear transfer and transgenesis (Duszewska et al, 2012;Hafez, 2015). Furthermore, it allows analyzing embryo development potential, including the pattern of gene expression, epigenetic modifications and cytogenetic disorders during the development (Galli & Lazzari, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it allows analyzing embryo development potential, including the pattern of gene expression, epigenetic modifications and cytogenetic disorders during the development (Galli & Lazzari, 2008). The technology generally refers to a number of procedures performed in the laboratory which includes maturation, fertilization, and culture procedures required to produce embryos from immature oocytes (Duszewska et al, 2012). The first successful IVF was achieved on rabbits in 1959 (Chang, 1959), followed by mice in 1968, human in 1978 (Steptoe, 1980) and the first born calf produced with IVF was in 1981 (Brackett et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

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145 the Role of Cell Apoptosis on in Vitro-Produced Beef Cattle Embryos

Nkadimeng

2017

Reprod. Fertil. Dev.

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Successful in vitro development of embryos is dependent upon maintenance of cellular function in the embryonic microenvironment. Exposure of pre-implantation embryos to a variety of cellular stresses can induce apoptosis in all or a fraction of blastomeres. Among the conditions that can induce apoptosis in cattle embryo production in vitro are heat shock, media composition, oxygen levels, and tumour necrosis factor (TNF)-α. The aim of the study was therefore to evaluate the in vitro culture environment of embryos in terms of cell apoptosis through caspase and DNA fragmentation evaluation. A total of 1200 immature oocytes were collected at slaughter from indigenous South African cow ovaries. The cumulus-oocyte complexes were randomly allocated into 2 maturation temperatures, 39°C and 41°C (200/temperature /replicated 6 times), and cultured in M199 + FSH-LH-oestradiol medium under oil at 100% humidity and 5% CO2 for 24 h. Postmaturation, oocytes were subjected to normal subsequent embryo conditions (Rynkowska et al. 2011 Biotechnol. Comput. Biol. Bionanotechnol. 92, 45–53). All matured oocytes were fertilized for 6 h with frozen-thawed Nguni bull semen from 10 bulls replicated 5 times at a concentration of 265 × 106 sperm cells/mL in Bracket and Oliphant medium under oil. The presumptive zygotes from each treatment were cultured into SOF-BSA medium under oil and incubated at 39°C for assessment of cleavage rate post IVF. Produced embryos were divided into 3 groups: 2–4 cell, ≥8 cell embryos, and blastocyst. Two- to four-cell embryos were removed at Day 2, ≥8 cell at Day 5 (mainly 8-cell, morula, and cavitated blastocyst), and expanded blastocyst at Day 7 of embryo culture to determine caspase activity and DNA fragmentation for evidence of apoptosis. Caspase was performed using colourimetric assay on a 96-well microplate reader and monitored at 450 nm reference filter. The DNA fragmentation was examined using the TUNEL assay method and imaging was done using the Alexa Fluor® 488 emission at 519 nm. Data were analysed using SAS 9.2 software (SAS Institute Inc., Cary, NC, USA), and Shapiro-Wilks test was used on the standardized residuals to test for deviations from normality, means of significant effects were compared using Student’s t-l.s.d. at the 95% confidence interval. There was no significant difference on the cleavage rates [39°C (72.0 ± 22.70) and 41°C (67.2 ± 18.9)] and blastocysts [39°C (11.4 ± 2.6) and 41°C (11.2 ± 6.3)] from both maturation temperatures. Caspase activity showed no significant difference on 2–4 cell [39°C (0.015 ± 0.001) and 41°C (0.016 ± 0.002)] and ≥8 cell embryos at both temperatures [39°C (0.022 ± 0.007a) and 41°C (0.032 ± 0.013)]. However, blastocyst differed significantly at both temperatures [39°C (0.037 ± 0.012) and 41°C (0.053 ± 0.005)]. A higher (P < 0.05) DNA fragmentation was observed at 2–4 cell (6.5 ± 2.9), ≥8 cell (13.3 ± 3.1), and blastocyst (48.0 ± 8.2) for embryos produced from 41°C matured oocytes compared to the 39°C maturation group [2–4 cell (2.2 ± 1.2), ≥8 cell (4.5 ± 1.9), and blastocyst (9.7 ± 6.7)]. It is therefore concluded that embryos produced from 41°C matured oocytes can have the same developmental capacity as the 39°C maturation group. Moreover, both temperatures can show signs of apoptosis, however, with more apoptosis evidence in the 41°C than the 39°C maturation group.

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“…Programmes for protection of the Wisent “in situ” and “ex situ” have been implemented in many European countries (http://www.bison-ebcc.eu). A new approach, which allows us to actively protect the Wisent and help its preservation by increasing reproductive potential, is in vitro production of embryos (Duszewska, Rąpała, Trzeciak, Dąbrowski, & Piliszek, ).…”

Section: Introductionmentioning

confidence: 99%

Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species

Duszewska

Gręda

Baraniewicz

et al. 2018

Reprod Domestic Animals

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Wisent, or European bison (Bison bonasus), is listed as "vulnerable" on the IUCN Red List of Threatened Species and is therefore protected by international law. For the first time, a Wisent embryo has been obtained in vitro. This procedure creates a new opportunity to protect and increase Wisent reproductive potential and thereby opens new possibilities for the establishment of a controlled and broad reserve of the gene pool.

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Obtaining farm animal embryos <i>in vitro</i>

Cited by 6 publications

References 92 publications

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

145 the Role of Cell Apoptosis on in Vitro-Produced Beef Cattle Embryos

Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species

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